Alcalase, Flavorzyme, Neutrase, and Protamex were purchased from Novozymes (Bagsvaerd, Denmark). Bromeline and Papain were purchased from Daesong Sangsa (Seoul, Korea). All chemicals for antioxidant and anti-aging tests were purchased from the Sigma-Aldrich Chemical Company (St. Louis, MS, USA). All other reagents and solvents used in this study were of analytical grade.
Flavorzyme
Manufactured by Novozymes
Sourced in Denmark
Flavorzyme is a microbial enzyme preparation that can be used to enhance flavors in food and beverage products. Its core function is to catalyze the breakdown of proteins and peptides, which can release amino acids and other flavor compounds that contribute to the overall taste and aroma of the final product.
Automatically generated - may contain errors
Lab products found in correlation
Alcalase, by Novozymes (4 mentions)
Protamex, by Novozymes (2 mentions)
Neutrase, by Novozymes (1 mentions)
2 4 6 trinitrobenzenesulfonic acid tnbs, by Merck Group (1 mentions)
Il 13, by Thermo Fisher Scientific (1 mentions)
Mouse ifn γ, by Thermo Fisher Scientific (1 mentions)
Mmcp 1, by Thermo Fisher Scientific (1 mentions)
Pe anti mouse il 4, by BD (1 mentions)
Cholera toxin, by Merck Group (1 mentions)
4 protocols using flavorzyme
1
Enzymatic Hydrolysis of Proteins
2
Skate Skin Hydrolysis and Deodorization Protocol
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The skin of a skate (R. kenojei) was frozen and used as raw material. It was defrosted and washed with running tap water, then extracted with high pressure for 1.5 h at 121 °C following the addition of water to the pressure tank. After freezing at -50 °C for 2 h and adding 0.1% Alcalase (Novozymes, Bagsvaerd, Denmark) and Flavorzyme (Novozymes), the skin sample was hydrolyzed for 2 h and then deodorized for 30 min at 75 °C using activated carbon, followed by filtering and concentrating at 60 °C under reduced pressure. After freezedrying, a CPSS was obtained as the final raw material. The CPSS was prepared immediately before treatment by suspending it in sterile distilled water for treatment.
3
Enzyme-assisted Protein Hydrolysis and GABA Assay
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Alcalase and Flavorzyme were purchased from Novozymes (Bagsvaerd, Denmark); Foodpro PNL, Protamex, and Prozyme 2000P were procured from Bision Biochem (Sungnam, Korea), and Collupulin was obtained from DSM Food Specialties (Delft, The Netherlands). γ-Aminobutyric acid (GABA), picrotoxin (PIX), bicuculline (BIC), flumazenil (FMZ), and 2,4,6-trinitrobenzene sulfonic acid (TNBS) were bought from Sigma-Aldrich (St. Louis, MO, USA).
4
Murine Allergy Model Protein Characterization
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Fresh CM was obtained from a local farm in Shenyang, China. Alcalase (EC 3.4.21.62), Protamex (EC 3.4.24.28), and Flavorzyme (EC 3.4.11.1) were purchased from Novozymes (Novo Nordisk). Cholera toxin was purchased from Sigma-Aldrich Chemical Co. Mouse IgE and IgG ELISA kits, as well as mouse IFN-γ, IL-4, IL-5, IL-13, IL-10, and mMCP-1, were obtained from eBioscience. Mouse histamine ELISA kits were acquired from Demeditec Company. Peridinin chlorophyll protein complex (PerCP) anti-mouse CD3e, fluorescein isothiocyanate (FITC) anti-mouse CD4, phycoerythrin (PE) anti-mouse CD25, allophycocyanin (APC) anti-mouse Foxp3, PE anti-mouse IL-4, and PerCP anti-mouse IFN-γ antibodies were purchased from BD Pharmingen. All chemicals used were of analytical grade.
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