Trans resveratrol 70675 res

Manufactured by Cayman Chemical

Sourced in United States

Trans-resveratrol (70675) (RES) is a naturally occurring polyphenolic compound. It is a colorless crystalline solid.

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Lab products found in correlation

2 aminoethyl diphenylborinate a16606 2 apb, by Thermo Fisher Scientific (2 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide ac158990050 mtt, by Thermo Fisher Scientific (2 mentions) Mda mb 231 human breast cancer htb 26 cell line, by American Type Culture Collection (1 mentions) Pc3 prostate cancer cell line, by American Type Culture Collection (1 mentions)

3 protocols using trans resveratrol 70675 res

Breast Cancer Cell Line Characterization

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The MDA-MB-231 human breast cancer (HTB-26) cell line was purchased from ATCC (Manassas, VA, USA). The passage numbers of cells used in experiments ranged from 5 to 20. thapsigargin (10522) (TG), fura-2-acetoxymethyl ester (14591) (Fura-2), N,N′-[1,2-ethanediylbis[(oxy-2,1-phenylene)]]bis[N-[2-(acetyloxy)methoxyl]-2-oxoethyl]-bis[(acetyloxy)methyl] ester (BAPTA-AM-Acetoxymethyl ester) (15551) (BAPTA-AM), and trans-resveratrol (70675) (RES) were purchased from Cayman Chemical (Ann Arbor, MI, USA). U-73122 (J62898) and 2-Aminoethyl diphenylborinate (A16606) (2-APB) were purchased from Alfa Aesar (Ward Hill, MA, USA). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (AC158990050) (MTT) was purchased from Acros Organics (Morris Plains, NJ, USA). 4′-Butyrate resveratrol (BuRV), 4′-Isobutyrate resveratrol (IsoRV) and 4′-Pivalate resveratrol (PIV) were synthesized by the Andrus lab in the Department of Chemistry and Biochemistry at Brigham Young University [18 (link)].

Peterson J.A., Doughty H.P., Eells A.J., Johnson T.A., Hastings J.P., Crowther C.M., Andrus M.B, & Kenealey J.D. (2017). The Effects of 4′-Esterified Resveratrol Derivatives on Calcium Dynamics in Breast Cancer Cells. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 22(11), 1968.

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Prostate Cancer Cell Line Characterization

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The PC-3 prostate cancer cell line was purchased from ATCC (Manassas, VA, USA). The passage numbers of cells used in experiments ranged from 5 to 20. Thapsigargin (10522) (TG), fura-2-acetoxymethyl ester (14591) (Fura-2), and trans-resveratrol (70675) (RES) were purchased from Cayman Chemical (Ann Arbor, MI, USA). U-73122 (J62898) and 2-Aminoethyl diphenylborinate (A16606) (2-APB) were purchased from Alfa Aesar (Ward Hill, MA, USA). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (AC158990050) (MTT) was purchased from Acros Organics (Morris Plains, NJ, USA). 4′-Butyrate resveratrol (BuRV), 4′-isobutyrate resveratrol (IsoRV) and 4′-pivalate resveratrol (PIV, Fig. 1) were synthesized by the Andrus lab in the Department of Chemistry and Biochemistry at Brigham Young University [29 (link)]. The purity of the compounds was determined to be greater than 98% by NMR and mass spectrometry.Fig. 1

Structures.

Structures of (A) trans-resveratrol, (B) 4′-pivalate trans-resveratrol, (C) 4′-isobutyrate trans-resveratrol, and (D) 4′-butyrate trans-resveratrol.

Fig. 1

Peterson J.A., Crowther C.M., Andrus M.B, & Kenealey J.D. (2019). Resveratrol derivatives increase cytosolic calcium by inhibiting plasma membrane ATPase and inducing calcium release from the endoplasmic reticulum in prostate cancer cells. Biochemistry and Biophysics Reports, 19, 100667.

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Calcium Signaling in Breast Cancer Cells

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The MDA-MB-231 human breast cancer (HTB-26) (MDA) and human primary dermal fibroblast (PCS-201–012) (PDF) cell lines were purchased from ATCC (Manassas, VA). The passage numbers of MDA cells used in experiments ranged from 10 to 35. Fura-2-Acetoxymethyl ester (14591) (Fura-2), thapsigargin (10522) (TG), (-)-epigallocatechin gallate (70935) (EGCG), quercetin (10005169), trans-resveratrol (70675) (RES), and BAPTA-Acetoxymethyl ester (15551) (BAPTA) were purchased from Cayman Chemical (Ann Arbor, MI). Lanthanum (III) chloride heptahydrate (LaCl₃) (262072) was purchased from Sigma-Aldrich (St. Louis, MO). Fura-2, pentasodium salt (50032) was purchased from Biotium (Hayward, CA).

Peterson J.A., Oblad R.V., Mecham J.C, & Kenealey J.D. (2016). Resveratrol inhibits plasma membrane Ca2+-ATPase inducing an increase in cytoplasmic calcium. Biochemistry and Biophysics Reports, 7, 253-258.

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