Ethylene diamine tetraacetic acid (edta)

Manufactured by Atlanta Biologicals

EDTA is a common laboratory reagent used as a chelating agent. It binds to metal ions, including calcium and magnesium, to prevent their interference in various analytical procedures. EDTA is widely used in clinical, analytical, and research applications to maintain sample integrity and ensure accurate results.

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Lab products found in correlation

B16f10 mouse melanoma cells, by American Type Culture Collection (1 mentions) Fetal bovine serum (fbs), by Atlanta Biologicals (1 mentions) Dulbecco s modification of eagle s medium, by Corning (1 mentions) Penicillin, by Atlanta Biologicals (1 mentions) Streptomycin, by Atlanta Biologicals (1 mentions) Trypsin, by Atlanta Biologicals (1 mentions)

2 protocols using ethylene diamine tetraacetic acid (edta)

B16.F10 Mouse Melanoma Cell Culture

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B16.F10 mouse melanoma cells (ATCC, Manassas, VA) were maintained in McCoy’s medium supplemented with 10% FBS and 1% gentamycin at 37°C and 5% CO₂ humidified air. Cells were removed from flasks using trypsin without EDTA (Atlanta Biologicals, Lawrenceville, GA) and washed in sterile DPBS without calcium and magnesium (Mediatech, Cellgro, Manassas, VA). Cell viability was assessed by trypan blue exclusion dye method. Cells with a viability >90% were resuspended in sterile DPBS at a concentration of 2 × 10⁷ per ml for injection.

Marrero B., Shirley S, & Heller R. (2014). Delivery of Interleukin-15 to B16 Melanoma by Electroporation Leads to Tumor Regression and Long-term Survival. Technology in Cancer Research & Treatment, 13(6), 551-560.

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MDCK Cell Culture Protocol

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MDCK cell strain-2 obtained from ATCC (Rockville, MD, USA) was grown in 75 cm² Corning polystyrene flasks (Corning, NY, USA). Dulbecco’s modification of Eagle’s medium (Cellgro, Manassas, VA, USA), which was enriched with 10% fetal bovine serum (Atlanta Biologicals, Lawrenceville, VA, USA), 100 units/mL penicillin, 100 μg/mL streptomycin (Atlanta Biologicals), and 1.42 g/L HEPES sodium was used to culture the cells. The cells were incubated at 37°C, 95% humidity, and 5% CO₂. The medium was changed three times a week, and the cells were trypsinized with 0.25% trypsin in 1.0 mM EDTA (Atlanta Biologicals). One-tenth of the harvest cells were seeded in a new polystyrene flask. The MDCK cells used were between passage number 45 and 65.

Laksitorini M.D., Kiptoo P.K., On N.H., Thliveris J.A., Miller D.W, & Siahaan T.J. (2015). Modulation of Intercellular Junctions by Cyclic-ADT Peptides as a Method to Reversibly Increase Blood-Brain Barrier Permeability. Journal of pharmaceutical sciences, 104(3), 1065-1075.

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