Mouse igg elisa kit

Buffers, substrates, and standards were prepared in accordance with the Cayman Chemical Mouse IgG ELISA Kit pre-assay preparation (Cayman Chemical 501240). Complete ELISA procedure was conducted according to the Cayman Chemical Mouse IgG ELISA Kit instructions.

pHrodo Green E. coli, PE anti-mouse/human Ki-67 antibodies, PE Rat IgG2b k Isotype Ctrl, and IgE Mouse ELISA Kit were purchased from Thermo Scientific (Waltham, MA, USA). Span^® 85 (S7135), TWEEN^® 80 (S7135), Deuterium oxide (151882), Culture media DMEM and RPMI 1640, L-glutamine, Pokeweed Mitogen (PWM), phytohemagglutinin (PHA), cytochalasin D, Methyl Methanesulfonate, Saponin, ELISA kits Mouse IL-2 ELISA Kit, TWEEN^® 20, Human Serum Albumin, and other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA). Turpentine Oil (State Standard 1571-82, Top Grade RPC 24 1611 0120) was purchased from «IB Smirnova» (Moscow, Russia). Drabkin Method Kit was purchased from Agat-Med Ltd. (Moscow, Russia). EasySep™ Mouse Monocyte Isolation Kit was purchased from StemCell Technologies Inc. (Vancouver, BC, Canada). Mouse IFN-gamma Quantikine ELISA Kit was purchased from R&D Systems Inc. (Minneapolis, MN, USA). Mouse TNF alpha ELISA Kit, Mouse IgM ELISA Kit, and Abcam Histamine ELISA kit were purchased from Abcam (Cambridge, UK). Mouse IgG ELISA Kit was purchased from Cayman Chemical (Ann Arbor, MI, USA). All other reagents used were of analytical reagent quality.