The Drosophila embryo used in this study (Fig. 2 ) expressed histone tagged with EGFP (w; His2Av::eGFP; Bloomington stock #23560). The embryos were collected by putting adult flies on a grape-juice agar plate for 45 min–1 h. After incubation at 25 °C for 1 h, the embryos were attached to a glass slide with double-sided tape. We use forceps to carefully roll an embryo on the tape until the embryo dechorionated. The Dechorionated embryos were embedded in 2% low-melting-temperature agarose in a Glass Bottom Dish (35 mm Dish with 20 mm Bottom Well, Cellvis). We put the Glass Bottom Dish on the microscope stage and scan the embryo along the z-axis 4 times with a 30 µm stride, then concatenate 4 reconstructed stacks to form the volume.
His2av egfp
Manufactured by Bloomington Drosophila Stock Center
His2Av::eGFP is a fluorescent protein fusion construct that labels the histone variant H2Av in Drosophila. This construct allows for the visualization and tracking of H2Av dynamics within the cell nucleus.
Automatically generated - may contain errors
2 protocols using his2av egfp
1
Drosophila Embryo Imaging Protocol
2
Drosophila Imaging Toolkit Protocols
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