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Green line

Manufactured by Tecniplast
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Sourced in Germany

The Green Line is an energy-efficient lab equipment solution designed to meet the needs of modern research facilities. It offers a range of core functionalities to support various laboratory workflows. The product is characterized by its energy-efficient design and commitment to sustainability.

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Lab products found in correlation

Balb canncrl balb c, by Charles River Laboratories (1 mentions) C57bl 6ncrl b6n, by Charles River Laboratories (1 mentions) Female c57bl 6j mice, by Charles River Laboratories (1 mentions) Nod cg prkdcscid il2rgtm1wjl szj mice, by Charles River Laboratories (1 mentions)

7 protocols using green line

1

Conditional Ext1 Knockout in Cartilage

Cited 1 time
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Transgenic Col2-rtTA-Cre;Ext1e2fl/e2fl [Tg(Col2a1-rtTA,tetO-cre) 22Pjro; Ext1tm1.1Vcs]6 (link), R26R-LacZ [Gt(ROSA)26Sortm1Sor]17 (link); Ndst1fl/fl mice [Ndst1tm1Grob]15 (link), Prx1-Cre [Tg(Prrx1-cre)1Cjt]18 (link) and Col2-Cre [Tg(Col2a1-cre)1Star]19 (link) mice were maintained on a C57Bl/6J genetic background. Up to six females, one breeding pair or single males were kept in air filtered cages (SPF conditions, Green Line, Techniplast) under a 12 h light/dark cycle on regular bedding with nesting material (Abed) as environmental enrichment and food (Sniff) and water ad libidum.
Genotyping was performed by PCR on tail tip DNA (DirectPCR Lysis Reagent; VIAGEN) with primers listed in Table S1. Allele recombination in Col2-rtTA-Cre;Ext1e2fl/e2fl mice was induced by peritoneal injection of 80 mg Doxycycline per kg body weight (kgbw) into lactating dams at postnatal day 8 (P8)6 (link). Mice undergoing surgery received 1 mg/kgbw Doxycycline at 6 weeks.
Severmann A.C., Jochmann K., Feller K., Bachvarova V., Piombo V., Stange R., Holzer T., Brachvogel B., Esko J., Pap T., Hoffmann D, & Vortkamp A. (2020). An altered heparan sulfate structure in the articular cartilage protects against osteoarthritis. Osteoarthritis and cartilage, 28(7), 977-987.
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2

Housing and Acclimation of C57BL/6N and BALB/c Mice

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Male C57BL/6NCrl (B6N) and BALB/cAnNCrl (BALBc) were obtained from Charles River (Bad Sulzfeld, Germany) at an age of 6 weeks and housed in groups of 3–4 in IVC cages (Greenline, Techniplast) under SPF conditions (23°C ± 4°C and 50% humidity ± 10%) with food and water ad libitum and a 12 h:12 h light-dark cycle (lights on: 08:00 h). Experiments were performed during the light phase of the light-dark cycle (i.e., between 09:00 and 17:00 h). The cages were equipped with bedding and rodent tunnel (4.5 cm × 4 cm, diameter: 30 mm; ABEDD, Vienna, Austria). Animals had at least 10 days to recover from transportation and to adapt to the new holding conditions before the experiments started. Animals of a given experiment were delivered and tested at the same time in order to keep environmental factors constant. In case of 1H-MRS imaging and WCM training, mice were isolated at least 1 week before starting the experiments and individually housed thereafter. Otherwise, mice were kept in groups of 3–4. All experimental procedures have been approved by the Government of Upper Bavaria (ROB-55.2-2532.Vet_02-17-224). All efforts were taken to reduce animal numbers and to minimize animal suffering.
Ebert T., Heinz D.E., Almeida-Corrêa S., Cruz R., Dethloff F., Stark T., Bajaj T., Maurel O.M., Ribeiro F.M., Calcagnini S., Hafner K., Gassen N.C., Turck C.W., Boulat B., Czisch M, & Wotjak C.T. (2021). Myo-Inositol Levels in the Dorsal Hippocampus Serve as Glial Prognostic Marker of Mild Cognitive Impairment in Mice. Frontiers in Aging Neuroscience, 13, 731603.
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3

Conditional Ext1 Knockout in Cartilage

Cited 1 time
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Transgenic Col2-rtTA-Cre;Ext1e2fl/e2fl [Tg(Col2a1-rtTA,tetO-cre) 22Pjro; Ext1tm1.1Vcs]6 (link), R26R-LacZ [Gt(ROSA)26Sortm1Sor]17 (link); Ndst1fl/fl mice [Ndst1tm1Grob]15 (link), Prx1-Cre [Tg(Prrx1-cre)1Cjt]18 (link) and Col2-Cre [Tg(Col2a1-cre)1Star]19 (link) mice were maintained on a C57Bl/6J genetic background. Up to six females, one breeding pair or single males were kept in air filtered cages (SPF conditions, Green Line, Techniplast) under a 12 h light/dark cycle on regular bedding with nesting material (Abed) as environmental enrichment and food (Sniff) and water ad libidum.
Genotyping was performed by PCR on tail tip DNA (DirectPCR Lysis Reagent; VIAGEN) with primers listed in Table S1. Allele recombination in Col2-rtTA-Cre;Ext1e2fl/e2fl mice was induced by peritoneal injection of 80 mg Doxycycline per kg body weight (kgbw) into lactating dams at postnatal day 8 (P8)6 (link). Mice undergoing surgery received 1 mg/kgbw Doxycycline at 6 weeks.
Severmann A.C., Jochmann K., Feller K., Bachvarova V., Piombo V., Stange R., Holzer T., Brachvogel B., Esko J., Pap T., Hoffmann D, & Vortkamp A. (2020). An altered heparan sulfate structure in the articular cartilage protects against osteoarthritis. Osteoarthritis and cartilage, 28(7), 977-987.
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4

Animal Models for Infectious Disease Research

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One-day-old chickens, 1-day-old turkeys, ferrets as well as C57BL/6 (B6) mice and human MxA transgenic (hMxAtg/tg) mice were used for this study. Chicks were bread at the FLI from SPF-chicken eggs (VALOBioMedia, Germany) and 1-day-old turkeys were ordered and shipped on hatching day from a local breeding facility (Bösel) to the FLI. The ferrets were obtained from the in-house breeding program at the FLI. B6 mice were obtained from Janvier and hMxAtg/tg mice were bred in-house at the Institute of Virology, Freiburg. All mice were housed in individually ventilated cages (Green Line, Tecniplast) with stable bedding (2HK: TAPVEI® BEDDING), at a temperature of 20.2 ± 1.1 °C, a humidity of 55% ± 7.1% and a 12 h:12 h light:dark cycle. The mice were fed ad libitum with food made from natural ingredients (Germany, altromin 1314). All animal work was performed in BSL3 containment facilities.
Halwe N.J., Hamberger L., Sehl-Ewert J., Mache C., Schön J., Ulrich L., Calvelage S., Tönnies M., Fuchs J., Bandawane P., Loganathan M., Abbad A., Carreño J.M., Bermúdez-González M.C., Simon V., Kandeil A., El-Shesheny R., Ali M.A., Kayali G., Budt M., Hippenstiel S., Hocke A.C., Krammer F., Wolff T., Schwemmle M., Ciminski K., Hoffmann D, & Beer M. (2024). Bat-borne H9N2 influenza virus evades MxA restriction and exhibits efficient replication and transmission in ferrets. Nature Communications, 15, 3450.
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5

Murine Model for Networked Cage Housing

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Female C57Bl/6J mice (n = 60) were obtained from Charles River Laboratories (France). They were housed at a 12/12 light–dark cycle, and food and water were available ad libitum, first in conventional cages until the age of 11 weeks, or single-housed after implanting catheter and EMG electrodes to avoid implant damage by cage-mate biting. Cages with inserted dividers (green line, Tecniplast, Buguggiate, Italy) were used, allowing interaction by sound and smell and reducing social deprivation, complying with the 3R principles of animal experimentation.
von Siebenthal M., Akshay A., Besic M., Schneider M.P., Hashemi Gheinani A., Burkhard F.C, & Monastyrskaya K. (2023). Molecular Characterization of Non-Neurogenic and Neurogenic Lower Urinary Tract Dysfunction (LUTD) in SCI-Induced and Partial Bladder Outlet Obstruction Mouse Models. International Journal of Molecular Sciences, 24(3), 2451.
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6

Housing Conditions for NOD.Cg-Prkdc Mice

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NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ mice were purchased from Charles River. All animal experiments were performed in accordance with protocols approved by the Service de la Consommation et des Affaires Vétérinaires of Canton de Vaud, Switzerland (VD 1865.3, 1865.4, and 1865.5). Mice were maintained and handled according to Swiss guidelines for animal safety with a 12-h-light-12-h-dark cycle, controlled temperature and food and water in polysulfone bottles ad libitum in SPF conditions, cages enriched with nesting material, cardboard, and wood tunnels, 12 h light cycle, 7 a.m. to 7 p.m. Animals are housed in IVC polysulfone cages—Green line—from Tecniplast®, type II long. Bedding is provided by Aspen Tapvei® (little squares about 4 mm × 4 mm × 1 mm). Water is acidified (pH between 2.5 to 3) on a resin column (Prominent® CH system). Diet from Provimi-Kliba® (cat# 3242, irradiated). Housing room temperature is 22 ± 2 °C, humidity 55 ± 10%.
Scabia V., Ayyanan A., De Martino F., Agnoletto A., Battista L., Laszlo C., Treboux A., Zaman K., Stravodimou A., Jallut D., Fiche M., Bucher P., Ambrosini G., Sflomos G, & Brisken C. (2022). Estrogen receptor positive breast cancers have patient specific hormone sensitivities and rely on progesterone receptor. Nature Communications, 13, 3127.
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7

Housing Conditions for Mouse Studies

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Animals used in this study were group-housed with two to five mice in individually ventilated cages (Tecniplast Germany GmbH, Green Line or Blue Line) and kept under identical housing conditions: 12h light/dark cycle (light cycle 6:30 a.m. to 6:30 p.m.), standard pelleted mouse diet (ssniff GmbH, Soest, Germany, article number v1124-300) ad libitum, free access to water, 22 ± 2°C room temperature and relative humidity of 55 ± 10%. The cages contained wooden bedding material (Tapvei Estonia OÜ, Estonia, Aspen bedding, article number 4HK 10 kg), nestlets (ssniff GmbH, Soest, Germany, article number H3279-10), a red plastic house (ZOONLAB GmbH, Castrop-Rauxel, Germany) and paper tunnels (ZOONLAB GmbH, Castrop-Rauxel, Germany; article number 3084030) as cage enrichment. Animals were handled by male and female caretakers and technicians.
Jeuthe S., Kemna J., Kemna C.P., Zocholl D., Klopfleisch R., Palme R., Kirschbaum C., Thoene-Reineke C, & Kammertoens T. (2020). Stress hormones or general well-being are not altered in immune-deficient mice lacking either T- and B- lymphocytes or Interferon gamma signaling if kept under specific pathogen free housing conditions. PLoS ONE, 15(9), e0239231.
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