Proteins, peptides, caffeine, and water-18O (97% atom) were purchased from Sigma Aldrich. Phosphatidylcholine was purchased from Fujifilm. 10-Acetyl-3,7-dihydroxyphenoxazine (ADHP) was purchased from Cayman Chemical. Hydrochloric acid, ammonium formate, ammonium acetate, and tetraethylammonium bicarbonate were from Kanto Chemical (Tokyo, Japan). All chemicals were used without further purification. The electrical conductivity of the solution was measured using a conductivity meter (Mettler Toledo). The 18O content for the isotope-labeled samples was greater than 87% atom.
Ammonium acetate
Manufactured by Kanto Chemical
Sourced in Japan
Ammonium acetate is a crystalline, water-soluble compound. It is commonly used as a buffer solution in analytical chemistry and biochemistry laboratories.
Automatically generated - may contain errors
Lab products found in correlation
Acetonitrile, by Kanto Chemical (4 mentions)
Clothianidin, by Merck Group (3 mentions)
Dichloromethane formic acid, by Kanto Chemical (3 mentions)
Clothianidin d3, by Merck Group (3 mentions)
Dinotefuran d3, by Merck Group (3 mentions)
Thiacloprid d4, by Merck Group (3 mentions)
Imidacloprid d4, by Merck Group (3 mentions)
Thiamethoxam d4, by Merck Group (3 mentions)
Thiamethoxam, by LGC (3 mentions)
Dinotefuran, by Kanto Chemical (2 mentions)
Imidacloprid, by Kanto Chemical (2 mentions)
Nitenpyram, by Kanto Chemical (2 mentions)
Thiacloprid, by Kanto Chemical (2 mentions)
Acetamiprid, by Kanto Chemical (2 mentions)
N desmethylacetamiprid, by Merck Group (2 mentions)
Hydrochloric acid (hcl), by Kanto Chemical (2 mentions)
Ammonium formate, by Kanto Chemical (1 mentions)
Water 18o, by Merck Group (1 mentions)
Caffeine, by Merck Group (1 mentions)
Conductivity meter, by Mettler Toledo (1 mentions)
8 protocols using ammonium acetate
1
Isotope-Labeled Biomolecule Analysis
2
Gut Microbiome Metabolic Profiling
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The B4PC2 consortium consisted of Bacteroides uniformis ATCC 8492, Bacteroides vulgatus ATCC 8482, Clostridium hylemonae DSM 15053, Clostridium hiranonis DSM 13275, Parabacteroides distasonis DSM 20701, Bilophila wadsworthia DSM 11045, and Blautia producta ATCC 27340. Strains were cultured and stored as previously described [12 ]. Authentic reference bile acids were described in our recent publication [12 ] and purchased from Sigma-Aldrich (St. Louis, MO) and internal standards were obtained from C/D/N Isotopes (Pointe-Claire, QC, Canada). Rare bile acids and sulfated-derivatives were gifts from Professor Iida, Nihon University, Tokyo, Japan (takaiiada@chs.nihon-u.ac.jp). Solvents (water, ethanol, methanol, acetonitrile) were of high-performance liquid chromatography grade, and ammonium acetate was analytical grade, all of which were purchased from Kanto Chemical (Tokyo, Japan).
3
Neonicotinoid Pesticide Quantification
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Acetamiprid, dinotefuran, imidacloprid, nitenpyram and thiacloprid were purchased from Kanto Chemical Corp. (Tokyo, Japan). Clothianidin, Clothianidin-d3, dinotefuran-d3, imidacloprid-d4, thiacloprid-d4, Thiamethoxam-d4, and N-desmethylacetamiprid (DMAP) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Hydroxyimidacloprid was purchased from Hypha Discovery (Slough, UK). Acetamiprid-d6 and nitenpyram-d3 were purchased from Hayashi Pure Chemical Ind. (Osaka, Japan). Thiamethoxam was purchased from Dr. Ehrenstorfer. Acetonitrile, dichloromethane formic acid, ammonium acetate and distilled water were all HPLC grade and were purchased from Kanto Chemical.
4
Neonicotinoid Pesticide Quantification
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Acetamiprid, dinotefuran, imidacloprid, nitenpyram and thiacloprid were purchased from Kanto Chemical Corp.
(Tokyo, Japan). Thiamethoxam was purchased from Dr. Ehrenstorfer. Clothianidin, Clothianidin-d3, dinotefuran-d3, imidacloprid-d4, thiacloprid-d4, Thiamethoxam-d4, and N-desmethylAcetamiprid (DMAP) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Acetamiprid-d6 and nitenpyram-d3 were purchased from Hayashi Pure Chemical Ind. (Osaka, Japan). Acetonitrile, dichloromethane formic acid, ammonium acetate and distilled water were all HPLC grade and were purchased from Kanto Chemical.
(Tokyo, Japan). Thiamethoxam was purchased from Dr. Ehrenstorfer. Clothianidin, Clothianidin-d3, dinotefuran-d3, imidacloprid-d4, thiacloprid-d4, Thiamethoxam-d4, and N-desmethylAcetamiprid (DMAP) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Acetamiprid-d6 and nitenpyram-d3 were purchased from Hayashi Pure Chemical Ind. (Osaka, Japan). Acetonitrile, dichloromethane formic acid, ammonium acetate and distilled water were all HPLC grade and were purchased from Kanto Chemical.
5
Quantification of Neonicotinoid Pesticides
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Acetamiprid, dinotefuran, imidacloprid, nitenpyram and thiacloprid were purchased from Kanto Chemical Corp. (Tokyo, Japan). Thiamethoxam was purchased from Dr. Ehrenstorfer. Clothianidin, Clothianidin-d3, dinotefuran-d3, imidacloprid-d4, thiacloprid-d4, Thiamethoxam-d4, and N-desmethylAcetamiprid (DMAP) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Acetamiprid-d6 and nitenpyram-d3 were purchased from Hayashi Pure Chemical Ind. (Osaka, Japan). Acetonitrile, dichloromethane formic acid, ammonium acetate and distilled water were all HPLC grade and were purchased from Kanto Chemical.
6
Adenosine and 15N5-adenosine Protocol
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Adenosine and 15 N5-Adenosine were purchased from Tokyo Chemical Industry Co., Ltd. and Silantes, respectively, and used as received without further purification. The LC-MS grades of acetonitrile and ammonium acetate were available from Kanto Chemical Co., Inc. and Fluka, respectively. Pooled human plasma was purchased from Kohjin Bio (Cat. Code: 12250110).
7
Sequential Extraction of Sediments
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Some deposited sediments were treated with the dithionite-citrate, acid ammonium oxalate, and acid hydroxylamine methods to remove amorphous inorganic particles (Carter and Gregorich 2007) . These treatments dissolve non-crystalline aluminosilicates, Fe oxides, and organically complexed metals, including Al, Fe, and Mn, via ligand-and reduction-promoted processes, although the methods are much less effective in removing crystalline Al. The treatments were conducted by using the standard protocol described elsewhere (Carter and Gregorich 2007) , except that excess SCs was amended in this study to the sample solution to prevent readsorption of dissociated RCs (SCs/SDW of ~2.0 μmol•g -1 and SDW/SV of 0.14 g•mL -1 ).
The dried sediments were treated at a SDW/SV ratio of ~0.75 g•mL -1 with 1 M HCl (Kanto Chemical), 1 M HNO3 (Kanto Chemical), 1 M NaOH (Kanto Chemical), 1 M ammonium acetate (Kanto Chemical), and MQ water. Again, in all cases, the solution was amended with SCs at final concentrations of ~200 μM, corresponding to SCs/SDW of 1.6-2.1 μmol•g -1 . Sediment suspensions were then incubated for an hour at 25 °C under gentle agitation. After centrifuging and decanting the supernatant, the pellets were washed three times by resuspending in MQ and centrifuging. The washing process was repeated for three times followed by the freeze drying.
The dried sediments were treated at a SDW/SV ratio of ~0.75 g•mL -1 with 1 M HCl (Kanto Chemical), 1 M HNO3 (Kanto Chemical), 1 M NaOH (Kanto Chemical), 1 M ammonium acetate (Kanto Chemical), and MQ water. Again, in all cases, the solution was amended with SCs at final concentrations of ~200 μM, corresponding to SCs/SDW of 1.6-2.1 μmol•g -1 . Sediment suspensions were then incubated for an hour at 25 °C under gentle agitation. After centrifuging and decanting the supernatant, the pellets were washed three times by resuspending in MQ and centrifuging. The washing process was repeated for three times followed by the freeze drying.
8
Pyrene Metabolism Profiling Protocol
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Pyrene, PYOH, 3'-phosphoadenosine 5'-phosphoslfate (PAPS), sulfatase (from limpet Type V; 34 U/mg), β-glucuronidase (from bovine liver, Type B-1; 1,240 U/mg), β-glucosidase (from almond; 3.4 U/mg), and bovine serum albumin were obtained from Sigma-Aldrich Co. (St.
Louis, MO). Methanol, acetonitrile, acetic acid, and ammonium acetate were purchased from Kanto Chemical Co., Inc. (Tokyo, Japan). Pyrene-1-sulfate (PYOS) was obtained from TOPU Bio (Toyama, Japan). Uridine-diphosphate-glucuronic acid (UDP-GA) was purchased from Wako (Osaka, Japan). All chemicals used for high-performance liquid chromatography (HPLC) and mass spectrometry (MS) were of HPLC or MS grade, respectively, and were obtained from Kanto Chemical Co. Inc.
Louis, MO). Methanol, acetonitrile, acetic acid, and ammonium acetate were purchased from Kanto Chemical Co., Inc. (Tokyo, Japan). Pyrene-1-sulfate (PYOS) was obtained from TOPU Bio (Toyama, Japan). Uridine-diphosphate-glucuronic acid (UDP-GA) was purchased from Wako (Osaka, Japan). All chemicals used for high-performance liquid chromatography (HPLC) and mass spectrometry (MS) were of HPLC or MS grade, respectively, and were obtained from Kanto Chemical Co. Inc.
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