The oil content of tocopherol and tocotrienol was determined using HPLC (Agilent 1100 apparatus) as described by Abdel-Razek et al. [6 ], using the same conditions. While, the sterol fractions of Moringa oil were analyzed according to the methodology of Stuper-Szablewska et al., [36 (link)], which was performed using an Aquity H class UPLC system equipped with a Waters Acquity photodiode array (PDA) detector (Waters, USA).
Acquity photodiode array pda detector
Manufactured by Waters Corporation
Sourced in United States
The ACQUITY photodiode array (PDA) detector is a high-performance liquid chromatography (HPLC) detector designed to analyze and detect a wide range of chemical compounds. The core function of the ACQUITY PDA detector is to provide sensitive, reliable, and accurate detection of analytes in complex samples across a broad wavelength range.
Automatically generated - may contain errors
Lab products found in correlation
Uplc h class system, by Waters Corporation (1 mentions)
H class uplc, by Waters Corporation (1 mentions)
Acquity ultra performance lc system, by Waters Corporation (1 mentions)
Acquity beh c18 column, by Waters Corporation (1 mentions)
Q exactive plus mass spectrometer, by Thermo Fisher Scientific (1 mentions)
Exactive plus emr mass spectrometer, by Thermo Fisher Scientific (1 mentions)
Acquity uplc classic system, by Waters Corporation (1 mentions)
Vanquish horizon uhplc system, by Thermo Fisher Scientific (1 mentions)
Synapt g2 si, by Waters Corporation (1 mentions)
Orbitrap fusion lumos mass spectrometer, by Thermo Fisher Scientific (1 mentions)
5 protocols using acquity photodiode array pda detector
1
Analyzing Moringa Oil Composition
2
Fungal Phenylpropanoid Quantification Protocol
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Fungal-derived phenylpropanoid concentrations were established in dried fungal cultures after 21 days of incubation (Supplementary Table S1 ), as previously described by Kulik et al. [28 (link),29 (link)] and Bilska et al. [30 (link)]. Alkaline and acid hydrolysis were conducted in sealed 17-mL culture tubes containing 0.2 g of fungal biomass. An ACQUITY H class UPLC (Ultra performance liquid chromatography) system equipped with a Waters ACQUITY Photodiode Array (PDA) detector (Waters, Milford, MA, USA) was used in the analysis. Concentrations of l -pyroglutamic acid were determined using an internal standard at a wavelength of λ = 310 nm. l -Pyroglutamic acid identification was based on a comparison of sample and standard retention times. A specific amount of the standard was added to the analyzed samples. In the next step, the analysis was repeated. The detection level was 1 μg/g. The retention time of l -pyroglutamic acid was 19.05 min.
3
UPLC Analysis of Medicinal Compounds
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Ultra-performance liquid chromatography) analysis was performed using an ACQUITY ultraperformance LC system equipped with ACQUITY photodiode array (PDA) detector (Waters Corporation, Milford, CT, USA) based on the methods described in a previous study [18 (link)]. ACQUITY BEH C18 column (Waters Corporation; 1.7 μm, 2.1 × 100 mm) was used to elute the compounds off and Empower software was applied to tract and compare the peak data. The standard compounds were melted by methanol and DMSO. Next, they were prepared based on a standard undiluted solution containing 1 mg/mL. The PDA analysis wavelengths were sennoside A (340 nm), emodin (254 nm), chrysophanol (254 nm), aloe-emodin (254 nm), rhein (254 nm), glycyrrhizin acid (254 nm), liquiritigenin (280 nm), and isoliquiritigenin (280 nm), respectively. Samples were 2 mL, and the flow rate was 0.4 mL/min. Five and three potential ingredients were collected from R. undulatum and G. uralensis, respectively (Figure 1 and Table 1 ).
4
IEX-MS Analysis of Deglycosylated mAbs
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Example 1
Methods
mAbs were subjected to online IEX-MS analysis. An aliquot of the deglycosylated mAb sample (˜50 μg) was injected onto a YMC-BioPro SP-F strong cation exchange (SCX) column (100×4.6 mm) coupled to a Thermo Exactive Plus EMR mass spectrometer or a Thermo Q Exactive plus mass spectrometer for mass measurement. The samples were separated and eluted over a 20 minute pH gradient with ammonium acetate based buffers (buffer A: 20 mM ammonium acetate, pH 5.8; buffer B: 200 mM ammonium acetate, pH 7.6). An analytical splitter (˜200:1 ratio) was connected after the SCX column to reduce the analytical flow to ˜2 μL/min prior to the mass spectrometer for mass detection. The high flow from the splitter was diverted to a Waters ACQUITY photodiode array (PDA) detector for simultaneous UV detection (280 nm).
Results
As a result, an acidic shoulder peak was detected and attributed to a variant of the antibody with a mass increase of approximately 176 Da. However, the mass measurement at the intact level was not accurate because of complications from a glycation modification which elutes in the same acidic shoulder peak and is close in mass (162 Da).
5
Mass Spectrometry Analysis of Compounds
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An Orbitrap Fusion
Lumos mass spectrometer (Thermo Fisher Scientific,
CA, USA) was coupled to a Vanquish Horizon UHPLC system with a VF-D40
detector (Thermo Fisher Scientific, CA, USA). A Synapt G2-Si (Waters,
UK) was coupled to an Acquity UPLC Classic system with the Acquity
Photodiode Array (PDA) detector (Waters, UK). Reverse-phase chromatography
was performed with the Acquity UPLC CSH C18 column, 1.0 × 150
mm, 1.7 μm (Waters, UK) using solvent A (0.1% formic acid in
water) and solvent B (0.1% formic acid in acetonitrile) as the mobile
phase. The remaining procedures and settings were standard.
Lumos mass spectrometer (Thermo Fisher Scientific,
CA, USA) was coupled to a Vanquish Horizon UHPLC system with a VF-D40
detector (Thermo Fisher Scientific, CA, USA). A Synapt G2-Si (Waters,
UK) was coupled to an Acquity UPLC Classic system with the Acquity
Photodiode Array (PDA) detector (Waters, UK). Reverse-phase chromatography
was performed with the Acquity UPLC CSH C18 column, 1.0 × 150
mm, 1.7 μm (Waters, UK) using solvent A (0.1% formic acid in
water) and solvent B (0.1% formic acid in acetonitrile) as the mobile
phase. The remaining procedures and settings were standard.
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