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Tempmodule system

Manufactured by Zeiss
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The TempModule system is a specialized lab equipment designed for precise temperature control and monitoring. It provides accurate and stable temperature regulation for various applications in research and testing environments.

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Lab products found in correlation

Plan apochromat 1.4 n a oil objective, by Zeiss (2 mentions) Lsm 880 airyscan confocal microscope, by Zeiss (2 mentions) Zen 2.3 blue edition, by Zeiss (1 mentions) Zen 2 blue edition, by Zeiss (1 mentions)

2 protocols using tempmodule system

1

Fluorescence Recovery After Photobleaching

Check if the same lab product or an alternative is used in the 5 most similar protocols
The treated cells were plated into 35 mm glass-bottom confocal dishes (NEST, 801001), and the FRAP experiment was performed on the Zeiss LSM880 Airyscan confocal microscope with a ×63 Plan-Apochromat 1.4 NA oil objective. The Zeiss TempModule system was used to control the temperature (37 °C), the humidity, and the CO2 (5%) of the imaging system. After imaging for 3 frames, the cells were photo-bleached using 100% laser power with the 488 nm laser (iterations: 50, stop when intensity drops to 50%). The cells were then imaged again every 2 s. The images were analyzed and measured with ZEN 2.3 blue edition (Zeiss) and Image J v1.48.
Yu C., Lei X., Chen F., Mao S., Lv L., Liu H., Hu X., Wang R., Shen L., Zhang N., Meng Y., Shen Y., Chen J., Li P., Huang S., Lin C., Zhang Z, & Yuan K. (2022). ARID1A loss derepresses a group of human endogenous retrovirus-H loci to modulate BRD4-dependent transcription. Nature Communications, 13, 3501.
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2

Fluorescence Recovery After Photobleaching

Check if the same lab product or an alternative is used in the 5 most similar protocols
The treated cells were plated into 35 mm glass bottom confocal dishes (NEST, 801001), and the FRAP experiment was performed on the Zeiss LSM880 Airyscan confocal microscope with a 63x Plan-Apochromat 1.4 NA oil objective. The Zeiss TempModule system was used to control the temperature (37 °C), the humidity and the CO2 (5%) of the imaging system. After imaging for 3 frames, the cells were photo-bleached using 100% laser power with the 488 nm laser (iterations: 50, stop when intensity drops to 50%). The cells were then imaged again every two seconds. The images were analyzed and measured with ZEN 2 blue edition (Zeiss).
Yuan K., Yu C., Lei X., Chen F., Mao S., Lv L., Liu H., Hu X., Wang R., Shen L., Zhang N., Meng Y., Shen Y., Li P., Huang S., Shao H., Lin C., & Zhang Z. (2021). ARID1A loss derepresses human endogenous retrovirus-H to modulate BRD4-driven transcription.
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