LNs were enzymatically digested, depleted of CD45+ cells, stained with 10.1.1 extracellularly and CD107a (1D48, BioLegend), cathepsin L (EPR8011, Abcam), biotin-anti-rabbit (Vector labs) and streptavidin Dylite 550 (Thermo Scientific) intracellularly before being cytospun onto slides and mounted with Slow Fade Gold with DAPI. Images were taken using an Axio Imager 2 with Apotome (Carl Zeiss), and brightness and contrast were adjusted using Zen software (Carl Zeiss).
Biotin anti rabbit
Manufactured by Vector Laboratories
Biotin-anti-rabbit is a secondary antibody reagent used in various immunoassay techniques. It is a biotinylated antibody that specifically binds to rabbit primary antibodies. This reagent can be used to detect and visualize rabbit-derived target proteins or antigens in biological samples.
Automatically generated - may contain errors
2 protocols using biotin anti rabbit
1
Immunohistochemistry of Lysosomal Proteins
2
Immunofluorescent Analysis of LN Cells
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