Camera microscope

Paraffin-embedded lung tissues were sectioned, dewaxed, and hydrated. Proteinase K solution was added at room temperature for 15 min to remove tissue proteins. Then, PBS with 2% hydrogen peroxide was added to the lung tissue sections for 5 min at room temperature. Subsequently, two drops of TdT enzyme buffer were added to the lung tissue sections for 1–5 min at room temperature. Next, TdT enzyme reaction solution was added dropwise to the slices and allowed to react at 37°C for 1 h. Then, washing and termination reaction buffer preheated to 37°C was added and maintained at 37°C for 30 min. Thereafter, peroxidase-labeled antibody was dropped to the slices and allowed to react for 30 min at room temperature. Finally, the sections were treated with DAB solution for 3–6 min and restained with methyl green for 10 min. The tissues were then dehydrated, sealed, and photographed under a LEICA camera microscope (Wetzlar, Germany).

First, the lung tissue soaked in formalin was taken out. The tissue was trimmed, placed in an embedding box, and rinsed with running water for approximately 6 h. The tissue was placed in a dehydrator for 12 h to complete dehydration, and then it was taken out and embedded into paraffin. After the paraffin had completely solidified, tissue sections were prepared and dried at 60°C for 2 h. The dried tissue was first stripped of paraffin by using xylene and then treated with different concentrations of alcohol. Finally, the tissue was treated in distilled water. After dyeing with HE stain, the tissue was dehydrated in alcohol, made transparent by using xylene, and sealed with neutral gum. Finally, the morphological changes of the lung tissue were observed under a LEICA camera microscope (Wetzlar, Germany).