Male C3H mice of 8-week-old were obtained from Charles River Laboratories. All animal studies were carried out in compliance with the guidelines of Institutional Animal Care and Use Committee (IACUC) of University of Pennsylvania. We followed a randomized, prospective, and controlled animal model design according to all the recommendations of the ARRIVE (Animal Research: Reporting In Vivo Experiments) guidelines. Mice were group-housed in polycarbonate cages (five animals per cage) in the animal facilities with controlled temperature (23 °C ± 2 °C), 40–65% humidity, and a 12-h light/dark cycle. Mice were acclimatized for at least 1 week before the study, fed with a standard laboratory diet, and allowed ad libitum access to drinking water.
Male c3h mice
Manufactured by Charles River Laboratories
Sourced in Japan, China
Male C3H mice are a commonly used strain of laboratory mice. They are genetically inbred and have a well-defined genetic profile. These mice are a model organism for various biomedical research applications.
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Lab products found in correlation
Male wistar rats, by Charles River Laboratories (1 mentions)
Male c57bl 6, by Charles River Laboratories (1 mentions)
Male balb c, by Charles River Laboratories (1 mentions)
Cd1 male mice, by Charles River Laboratories (1 mentions)
C57bl 6j, by Charles River Laboratories (1 mentions)
Balb c mice, by Charles River Laboratories (1 mentions)
6 protocols using male c3h mice
1
Controlled Mouse Housing and Acclimation
2
Rat and Mouse Animal Study Guidelines
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This study was approved by the Animal Care and Use Committee of Kagawa University (permission number: 2013–45) and the experiment was conducted in accordance with the Institutional Guidelines and Rules for Care and Use of Laboratory Animals. Male Wistar rats (n=30), weighing 280–320 g, and male C3H mice (n=10), weighing 28–32 g, were purchased from Charles River Laboratories Japan, INC. (Yokohama, Japan) and housed for at least 7 days in a clean room in the animal facility of the Faculty of Medicine, Kagawa University. The rats were given free access to commercial chow and water, and were maintained according to the Kagawa University animal guidelines.
3
Standardized C3H Mice Husbandry
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Male C3H mice (7 weeks old, 16-18 gram) were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China). All experimental procedures followed the guidelines of The Animal Ethics Committee of Zhejiang Chinese Medical University (Approval lot: ZSLL-2016-138) and The National Guidelines for Experimental Animal Welfare.
4
Coxsackievirus B3 Infection Model in Mice
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This study was approved by the Institutional Animal Research Committee of Tongji Medical College. All animal experimental protocols complied with the Guide for the Care and Use of Laboratory Animals published by the National Institutes of Health. Male A/J (~6-week-old) mice were obtained from GemPharmatech (Nanjing, China). Male C57BL/6 (~6-week-old) mice, male BALB/c (~6-week-old) mice, and male C3H mice were purchased from Beijing Vital River Laboratory Animal Technology (Beijing, China). All four mice strains were randomly assigned to two groups: control (n = 10) and CVB3-treated (n = 15) groups. A/J mice and BALB/c mice were intraperitoneally injected with 104 PFU CVB3, while C57BL/6 and C3H mice were intraperitoneally injected with 105 PFU CVB3, as described previously (Huber et al., 2001 (link); Althof et al., 2018 (link)). Phosphate-buffered saline (PBS) was used as the control. All animals were anesthetized with intraperitoneal injections of a mixture of xylazine (5 mg/kg) and ketamine (80 mg/kg). Echocardiography was performed on day 7 after virus injection, and the mice were sacrificed. Subsequently, the organs were collected and frozen in liquid nitrogen, followed by storage at −80°C.
5
Social Defeat Stress Experiment in Mice
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All of the animal procedures were approved by the Institutional Animal Care and Use Committees of Kyoto University and Yamaguchi University, and performed in accordance with the Guidelines for Animal Care and Use of Yamaguchi University and Kyoto University. Experimental animals were either male C57BL/6J or BALB/c mice (Charles River, Japan), 8 weeks old at the time of experimentation. In addition, retired breeder CD1 male mice (Charles River, Japan) were used as aggressors in the social defeat stress procedure and in the social interaction test (SIT); however, they were not included in any analysis. Four to six-week-old C3H male mice (Charles River, Japan) were used in the free-moving SIT. C57BL/6J, BALB/c, and C3H mice were housed under standard conditions in groups of 4-5 animals; CD-1 mice were housed individually until the start of the experiment on a 12-h light/dark cycle with mouse chow and water ad libitum.
6
Hydrodynamic Delivery of HBV Minicircle DNA
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All animal care and procedures were carried out according to the relevant National Institutes of Health guidelines and were approved by the Institutional Animal Care and Use Committee and the Office of Laboratory Animal Research at Charles River Accelerator and Development Laboratory. C3H male mice of 5–6 weeks of age were purchased from Charles River Laboratories. HBV minicircle DNA was injected into C3H mice using hydrodynamic delivery as described.38 (link) Four weeks later, HDI mice were assessed for HBsAg and organized into the four groups for further treatment, as indicated in Figure 5 . Serum was collected every 7 days from the submandibular vein and used to assess the levels of HBsAg. Lipid nanoparticles were diluted in sterile 1× Tris-buffered saline and intravenously injected via tail vein. Animals were euthanized 42 days post-LNP injection.
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