Ef 100mm f2.8 l is usm macro lens

Samples of fungi were collected in two different areas of Brazil, one in the State of Goiás (by FFA) and another in the northeast part of the State of São Paulo (by CJB and SSO). Both these areas have a biome classified as Semi-deciduous Forest, with a dry season from June to September. The fungi samples with mycetophilid larvae were transferred to plastic jars covered by a fine mesh. Samples were misted daily with water to maintain humidity. Five adults emerged from the fungi. All material examined, with the exception of the holotype, was kept pinned, preserved in 80% alcohol, or on slides, and housed in the Diptera collection of the Museu de Zoologia da Universidade de São Paulo (MZSP), São Paulo, Brazil. The holotype of N.puncticoxa was examined at the Natural History Museum (NHM), London, UK (additional information on the holotype is available in Amorim and Oliveira 2013 (link)).
Methods for the preparation of specimens, photos and illustrations follow Oliveira and Amorim (2012) . Morphological terminology follows Søli (1997) and Amorim and Rindal (2007) for adults and Courtney et al. (2000) for larvae. Field photos were taken with either a Canon EOS 5D Mark II camera with a Canon EF 100mm F2.8 L IS USM Macro lens and Canon Speedlite 580EX II Flash (FFA), or a Canon PowerShot SX200IS (CJB).

Photographs were taken at a distance of approximately 10 cm in “Raw” format with a Canon EOS 5D Mark V with ISO 800, shutter 1/100 and AV 18 using a Canon EF 100 mm f/2.8 L IS USM Macro Lens with manual focus. The white balance of “Raw” format photographs was changed to “Flash” using the Picture style editor software (Canon, Tokyo, Japan).
For each individual eye photograph, the eye color was determined quantitatively, using the Pixel Index of the Eye (PIE)‐score (Andersen et al. 2013).

Metazoan specimens were collected opportunistically from ten coral reef sites across Singapore from 2017 to 2019, either via intertidal surveys or subtidally via SCUBA. Collections were authorized by the National Parks Board (permit number NP/RP15-088), and samples were carefully treated according to NUS Institutional Animal Care and Use Committee (IACUC) guidelines (IACUC Protocol B15-1403) during the collection and vouchering process. During the vouchering phase, samples were grouped into phylum/class based on morphology. This was to facilitate downstream amino acid correction (see Section 2.5.), as well as morphology–barcode identity congruence checks. Voucher specimens were imaged using the Canon EF 100 mm f2.8/L IS USM macro lens on an EOS 750D.