Samples were analyzed on an Agilent 8890-5977B GC-MSD equipped with a
Pal3 autosampler that injected 1 μl of sample onto a VF-5MS (30 m x 0.25
mm x 0.25 mm) column. The samples were injected with a split ratio of 15:1,
helium flow rate of 1 ml/min and inlet temperature of 280 °C. The
temperature was held for 2 min at 125 °C, raised at 3 °C/min to
150 °C, 5 °C/min to 225 °C, and 15 °C/min to 300
°C and held for 1.3 min. The MSD was run in scan mode from 50-500 Da at a
frequency of 3.2 scan/s. Spectral deconvolution and compound identification was
performed with Masshunter Workstation Unknown Analysis software (Agilent) and
the NIST 2017 MS library. Best hits of compound identity are reported for
spectra with a match factor greater than 85%. Identified metabolites were then
manually mapped to metabolic pathways in the KEGG PATHWAY Database. Analyte
abundances were calculated using the MassHunter Workstation Quantitative
Analysis software (Agilent).
Pal3 autosampler that injected 1 μl of sample onto a VF-5MS (30 m x 0.25
mm x 0.25 mm) column. The samples were injected with a split ratio of 15:1,
helium flow rate of 1 ml/min and inlet temperature of 280 °C. The
temperature was held for 2 min at 125 °C, raised at 3 °C/min to
150 °C, 5 °C/min to 225 °C, and 15 °C/min to 300
°C and held for 1.3 min. The MSD was run in scan mode from 50-500 Da at a
frequency of 3.2 scan/s. Spectral deconvolution and compound identification was
performed with Masshunter Workstation Unknown Analysis software (Agilent) and
the NIST 2017 MS library. Best hits of compound identity are reported for
spectra with a match factor greater than 85%. Identified metabolites were then
manually mapped to metabolic pathways in the KEGG PATHWAY Database. Analyte
abundances were calculated using the MassHunter Workstation Quantitative
Analysis software (Agilent).