Curcumin was purchased from Sigma (St. Louis, MO). B14, a Curcumin analog (Figure 1 ), was provided by Dr. Ge RS (Department of Anesthesiology, the Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University).
In all in vitro experiments, Curcumin and B14 compounds were dissolved in medium containing 0.1% DMSO. Human breast cancer cell lines MCF‐7 and MDA‐MB‐231 were purchased from Shanghai Institute of Life Sciences Cell Resource Center (Shanghai, China). DMEM and FBS were obtained from Gibco BRL (Grand Island, NY, USA). MTT reagent was from Sigma (St. Louis, MO). The FITC Annexin V Apoptosis Detection Kit and Cell Cycle Staining Kit were purchased from BD Pharmingen (Franklin Lakes, NJ). GAPDH, Cyclin E1, Cyclin D1, Bax, Bcl‐2, Cyto‐C, TIMP1, Caspase3, cleaved Caspase3, and cleaved Caspase7 antibodies were purchased from Abcam company. Modified RIPA lysis buffer, protease inhibitor cocktail and chemiluminescent immunoblotting reagent were obtained from Thermo Fisher Scientific (Rockford, IL, USA).
In all in vitro experiments, Curcumin and B14 compounds were dissolved in medium containing 0.1% DMSO. Human breast cancer cell lines MCF‐7 and MDA‐MB‐231 were purchased from Shanghai Institute of Life Sciences Cell Resource Center (Shanghai, China). DMEM and FBS were obtained from Gibco BRL (Grand Island, NY, USA). MTT reagent was from Sigma (St. Louis, MO). The FITC Annexin V Apoptosis Detection Kit and Cell Cycle Staining Kit were purchased from BD Pharmingen (Franklin Lakes, NJ). GAPDH, Cyclin E1, Cyclin D1, Bax, Bcl‐2, Cyto‐C, TIMP1, Caspase3, cleaved Caspase3, and cleaved Caspase7 antibodies were purchased from Abcam company. Modified RIPA lysis buffer, protease inhibitor cocktail and chemiluminescent immunoblotting reagent were obtained from Thermo Fisher Scientific (Rockford, IL, USA).