Tissue extracts of colon homogenates were prepared as described in Cytokine measurements, diluted in Laemmli buffer and boiled. Samples were subjected to SDS-PAGE, and the proteins were transferred into PVDF membranes. After blocking (1% or 3% milk, 1-2h, RT), the membranes were stained using rabbit anti-mouse caspase-1 (SC514; Santa Cruz Biotechnology, Inc.) diluted 1:500, rat anti-mouse caspase-11 (Novus Biologicals; 1:1,000), rabbit anti-mouse IL-18 (Biovision; 1:1,000) and HRP-coupled anti-mouse β-actin (Novus Biologicals; 1:40,000). Membranes were exposed to the corresponding secondary antibodies (Jackson ImmunoResearch), washed and developed with ECL (GE Healthcare).
Rat anti mouse caspase 11
Manufactured by Novus Biologicals
Rat anti-mouse caspase-11 is a primary antibody that specifically binds to caspase-11, a member of the cysteine-aspartic acid protease (caspase) family, in mouse samples. Caspase-11 plays a role in the inflammatory response and cell death pathways.
Automatically generated - may contain errors
2 protocols using rat anti mouse caspase 11
1
Immunoblot Analysis of Inflammatory Mediators
2
Immunoblot Analysis of Inflammatory Mediators
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tissue extracts of colon homogenates were prepared as described in Cytokine measurements, diluted in Laemmli buffer and boiled. Samples were subjected to SDS-PAGE, and the proteins were transferred into PVDF membranes. After blocking (1% or 3% milk, 1-2h, RT), the membranes were stained using rabbit anti-mouse caspase-1 (SC514; Santa Cruz Biotechnology, Inc.) diluted 1:500, rat anti-mouse caspase-11 (Novus Biologicals; 1:1,000), rabbit anti-mouse IL-18 (Biovision; 1:1,000) and HRP-coupled anti-mouse β-actin (Novus Biologicals; 1:40,000). Membranes were exposed to the corresponding secondary antibodies (Jackson ImmunoResearch), washed and developed with ECL (GE Healthcare).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!