Mdc kit

Manufactured by Solarbio

Sourced in China

The MDC kit is a laboratory equipment designed for molecular detection and characterization. It provides the necessary components for nucleic acid extraction, amplification, and analysis. The kit includes reagents, buffers, and consumables required for these core functions.

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Lab products found in correlation

Confocal microscope, by Olympus (1 mentions)

Close spelling variants of this product

MDC staining kit (3 citations) Monodansylcadaverine (MDC) kit (3 citations) MDC assay kit (2 citations) Monodansylcadaverine assay kit (MDC, (2 citations)

3 protocols using mdc kit

Fluorescence Microscopy of MDC-Stained Cells

Cited 1 time

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According to the previous studies (Wang et al., 2019b (link); Wang et al., 2019c (link)), cells in each group were stained using the MDC kit (Solarbio, G0170) and observed under the fluorescence microscope (×400).

Zhang Y., Ma L., Lu E, & Huang W. (2021). Atorvastatin Upregulates microRNA-186 and Inhibits the TLR4-Mediated MAPKs/NF-κB Pathway to Relieve Steroid-Induced Avascular Necrosis of the Femoral Head. Frontiers in Pharmacology, 12, 583975.

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Autophagy Quantification in Liver Cells

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In order to investigate the autophagy in Huh7 and SMMC7721 cells, a MDC kit (Solarbio, China) was utilized according to the manufacturer's instruction. Briefly, 90 µL resuspended cells (1 × 10⁶ cells/mL) were stained with 10 µL MDC solution for 45 min at room temperature in the dark. Images were captured using a confocal microscope (Olympus, Japan), and MDC positive cells were counted.

He K., Liu X., Cheng S, & Zhou P. (2021). Zingiberensis Newsaponin Inhibits the Malignant Progression of Hepatocellular Carcinoma via Suppressing Autophagy Moderated by the AKR1C1-Mediated JAK2/STAT3 Pathway. Evidence-based Complementary and Alternative Medicine : eCAM, 2021, 4055209.

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Detecting Cellular Autophagy via MDC

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The autophagy rate of cells was detected by MDC staining. NIH-3T3 cells were suspended after digestion in the trypsin–EDTA solution, washed with a wash buffer, and resuspended again, followed by staining with the MDC staining solution at 37°C for 30 min. After washing with the wash buffer, the cells were resuspended in a collection buffer and then detected by flow cytometry. The MDC kit was purchased from Solarbio Biotechnology (China).

Chen C., Zhang H., Hou S., Liu Y., Ren L., Hao M., Chen K., Li L., Cai X., Deng Z., Liu Z, & Hou P. (2022). Shenxian-Shengmai Oral Liquid Evoke Autophagy of Fibroblast to Attenuate Sinoatrial Node Fibrosis in Sick Sinus Syndrome Mice via the AKT/mTOR Pathway. Evidence-based Complementary and Alternative Medicine : eCAM, 2022, 5219277.

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