The following drugs and reagents were procured for the experiments: Etomidate (Jiangsu Enhua Pharmaceutical Co.); 0.9% sodium chloride injection (Zhejiang DuBang Pharmaceutical Co.); TRIzol (Takara Bio Inc.); complete protein extraction kit (Beijing Solarbio Science & Technology Co., Ltd.); anti-Nrf2 protein antibody (Santa Cruz, USA); anti-HO-1 protein antibody (Santa Cruz, USA); anti-β-actin antibody (Santa Cruz Biotechnology, Inc.); PRIME-SCRIPT RT-PCR kit (Takara Bio Inc.); SYBR PreMix Ex kits (Takara Bio Inc.); tumor necrosis factor-α (TNF-α), interleukin (IL)-6, IL-1β and IL-10 ELISA kits (Shanghai Westang Biotechnology Co., Ltd.); myeloperoxidase (MPO), superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) kits (Nanjing Jiancheng Bioengineering Institute); and anti-mouse IgG antibody (Cell Signaling Technology, Inc.).
Complete protein extraction kit
Manufactured by Solarbio
Sourced in United States, China
The Complete Protein Extraction Kit is a comprehensive solution for the extraction and isolation of proteins from various biological samples. The kit includes all the necessary reagents and buffers to effectively lyse cells, solubilize proteins, and purify the extracted proteins. This kit provides a standardized and efficient method for protein extraction, suitable for a wide range of applications in biochemistry, molecular biology, and proteomics research.
Automatically generated - may contain errors
Lab products found in correlation
Primescript rt pcr kit, by Takara Bio (1 mentions)
Anti mouse igg antibody, by Cell Signaling Technology (1 mentions)
Sybr premix ex kit, by Takara Bio (1 mentions)
Trizol, by Takara Bio (1 mentions)
Anti β actin antibody, by Santa Cruz Biotechnology (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Pvdf membrane, by Beyotime (1 mentions)
Goat anti rabbit igg, by Huabio (1 mentions)
2 protocols using complete protein extraction kit
1
Etomidate-Induced Oxidative Stress Regulation
2
Protein Extraction and Western Blot Analysis
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Total protein was extracted from both ESCC cells and the cancerous tissue of ESCC patients utilizing the protocol of the complete protein extraction kit (Solarbio, China). After extraction, the protein was subjected to SDS-PAGE electrophoresis. This was followed by transferring the proteins onto a PVDF membrane (Millipore, USA), which was then blocked with a 5% skim milk solution in Tris-buffered saline/Tween (TBST). A primary antibody was then incubated overnight at 4 °C on the membrane. The following day, the PVDF membrane was cleansed with TBST (Beyotime, China) before it was incubated with goat anti-rabbit IgG (1:50000, HA1001, HUABIO) for 1 h at room temperature. Finally, the visibility of the proteins was enhanced using the right developing solution (Thermo Fisher Scientific, USA). Details about the utilized antibodies are available in the Supplementary Table 3 .
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