Uv vis evolution 60s

Manufactured by Thermo Fisher Scientific

Sourced in United States

The UV-VIS Evolution 60S is a spectrophotometer designed for UV-visible absorption and quantitative analysis. It offers a wavelength range of 190 to 1100 nm and a photometric range of -4 to 4 Abs. The instrument provides accurate and reliable data with a photometric accuracy of ±0.005 Abs and a wavelength accuracy of ±0.1 nm.

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Evolution 60S (76 citations) 60S Evolution (5 citations) Evolution 60S UV-Vis spectrophotometer (4 citations)

3 protocols using uv vis evolution 60s

Spectrophotometric TBARS Quantification

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To determine the TBARS concentration, a 0.37% solution of thiobarbituric acid TBA (AppliChem, Germany) in a 0.25 HCl molar solution (Chempur, Poland) was used in the test specimen.
A suspension of oxidised lipids was mixed with a TBA solution in the ratio of 1 : 1 and incubated in a water bath at 100°C for 10 minutes. Concurrently, a reference specimen was prepared (a mixture of deionised water and TBA solution in the ratio of 1 : 1). After incubation, the cooled specimens were transferred to cuvettes and, with the use of a spectrophotometer UV-VIS Evolution 60S (Thermo Scientific, USA), the absorbance at a wavelength of 535 and 600 nm was measured with respect to the reference specimen. The absorbance difference (A_diff = A₅₃₅ − A₆₀₀) is a measure of the concentration of TBARS in a specimen [22 (link)].
Next, a change in the value of the A_diff parameter in relation to the lipid control specimen (A₀), not subjected to oxidation (H₂O₂ concentration equal to 0 mM), was determined according to

\begin{matrix} Δ_{TBARS} = \frac{A_{diff} - A_{0}}{A_{0}} \cdot 100 % . \end{matrix}

A mean value of the parameter Δ_TBARS and standard deviation were determined for each value of H₂O₂ concentration from each seven measurement series.

Oleszko A., Olsztyńska-Janus S., Walski T., Grzeszczuk-Kuć K., Bujok J., Gałecka K., Czerski A., Witkiewicz W, & Komorowska M. (2015). Application of FTIR-ATR Spectroscopy to Determine the Extent of Lipid Peroxidation in Plasma during Haemodialysis. BioMed Research International, 2015, 245607.

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Evaluation of Antioxidant Capacity by Spectroscopic Methods

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2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 6-hydroxy-2,5,7,8-tetramethyl-chroman-2-carboxylic acid (Trolox), (2,4,6-tris (2-pyridyl)-1,3,5-triazine) (TPTZ) fluorescein (3‘,6‘-dihydroxyspiro[isobenzofuran-1[3H],9‘[9H]-xanthen]-3-one), 2,2-diphenyl-1-picrylhydrazyl (DPPH), were provided by Sigma–Aldrich Chemical Co. (St. Louis, MO, USA). Hydrochloric acid, ferrous sulfate, ferric chloride, methanol, and ethanol were provided by POCH (Gliwice, Poland). All the chemicals used were of analytical grade.
Spectrophotometric determinations were made using a spectrophotometer UV-VIS Evolution 60S (Thermo Scientific, USA). ORAC was performed in a Hitachi F-7000 spectrofluorimeter with an adapter for measurements on microplates (Hitachi High-Tech, Tokyo, Japan).

Cendrowski A., Kraśniewska K., Przybył J.L., Zielińska A, & Kalisz S. (2020). Antibacterial and Antioxidant Activity of Extracts from Rose Fruits (Rosa rugosa). Molecules, 25(6), 1365.

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Quantifying Shikimic Acid in Leaf Discs

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Eight leaf discs with a radius of 6 mm (~25 mg) were sampled from the second leaves from the apical meristem of each plant at 2, 6, 24, 48, 72, 120, 168, and 216 HAA to determine the concentration of shikimic acid. The leaf disc samples were kept in liquid N 2 during sampling and then stored at -80 °C until further analysis. Shikimic acid was extracted according to Singh and Shaner (1998) with some modifications. The frozen samples were ground in microtubes containing HCl (0.25 N) at a ratio 1:10 (tissue mass (g): volume of HCl 0.25 N (ml)). The extract was centrifuged at 15,000 × g for 25 min at 4 °C. After centrifugation, 30 μL of the supernatant was reacted with 500 μL of periodic acid (1%) for 45 min in a boiling water bath at 37 °C, and then 500 μL of sodium hydroxide (1 N) and 300 μL of glycine (0.1M) were added. The absorbances were measured at 380 nm in a spectrophotometer (UV-VIS Evolution 60S Thermo Fischer Scientific  , Madison, USA). The molar extinction coefficient of 4.76 × 10 4 M -1 cm -1 was used to calculate the shikimc acid concentration (Gaitonde and Gordon, 1958), which was expressed in mg g -1 of fresh weight (FW).

Batista P.F., Costa A.C., Megguer C.A., Lima J.S., Silva F.B., Guimarães D.S., Almeida G.M, & Nascimento K.J.T. (2018). Pouteria torta: a native species of the Brazilian Cerrado as a bioindicator of glyphosate action. Brazilian journal of biology = Revista brasleira de biologia, 78(2).

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