Phoresis software

Manufactured by Sebia

Sourced in France

Phoresis software is a core component of Sebia's electrophoresis equipment. It provides the necessary functionality to control and analyze the electrophoresis process.

Automatically generated - may contain errors

Lab products found in correlation

Hydragel agarose gels, by Sebia (2 mentions) Hydrasys densitometer, by Sebia (2 mentions) Hydrasys, by Sebia (2 mentions) Hydrasys 2, by Sebia (1 mentions) Perfection v700, by Epson (1 mentions) Hydrasys device, by Sebia (1 mentions)

5 protocols using phoresis software

VWF Multimer Analysis by Sebia Hydragel

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The VWF multimers method, developed by Sebia (France), is described in detail elsewhere [3] (link)
[4] (link). It was used by the participating laboratories without deviation from the original Sebia assay protocol. In brief, citrated plasma samples were analyzed on the Hydrasys 2 instrument (Sebia, France) with ready to use SDS agarose gels (Hydragel 5 von Willebrand multimers, Sebia). Densitometry of VWF multimer patterns was carried out with a transmission scanner (Sebia Gelscan Instrument) which allows scanning and data storage of the results. Data acquisition is performed by a bidimensional calibrated CCD sensor. The instrument, when connected to a PC with the Sebia PHORESIS software, allowed the operator to display the gel images, curves, curves overlapping, and quantification of multimer band patterns according to the manufacturer recommendation (LMWM 1-3 bands; IMWM 4-7 bands; and HMWM 8^th band and above).
The percentage values of each molecular weight multimer fraction was provided by the software. The calculation was made by applying the ratio of the area of each fraction and the total area under the curve. The multimer patterns of the plasma samples studied were, if necessary, compared with the reference pool pattern analyzed on the same gel. The total area under the curve of each sample was directly proportional to the amount of antigen (VWF:Ag).

Pikta M., Vasse M., Smock K.J., Moser K.A., van D.M., Lejniece S., Szanto T., Bautista H., Nouadje G, & Banys V. (2022). Establishing reference intervals for von Willebrand factor multimers. Journal of Medical Biochemistry, 41(1), 115-121.

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Electrophoretic Analysis of LDH Isoenzymes

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For the electrophoretic study, the samples of the liver and kidney extracts were used for each separation. A HYDRASYS device (Sebia, Lisses, France) was used to determine LDH isoenzyme activities according to protocols outlined in our previous study (Andrejčáková et al., 2016 (link), Andrejčáková et al., 2020 ). The samples were separated using commercial Hydragel 15 ISO-LDH electrophoretic kits (Ecomed, Žilina, Slovak Republic). Qualitative evaluations of gels were done directly from the electrophoretograms. Densitometric curves of the separations were created by means of Epson Perfection V 700 Photo densitometer scanning at λ 570 nm and evaluated using the PHORESIS software (Version 9.20, 2015, Sebia, Lisses, France).

Andrejčáková Z., Vlčková R., Sopková D., Kozioł K., Koziorowski M., Fabián D., Šefčíková Z., Holovská K., Almášiová V, & Sirotkin A.V. (2021). Dietary flaxseed's protective effects on body tissues of mice after oral exposure to xylene. Saudi Journal of Biological Sciences, 28(7), 3789-3798.

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Serum Protein Electrophoresis Profiling

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Blood was collected in Eppendorf by retro-orbital sampling. Semiautomated electrophoresis was performed on the Hydrasys instrument (Sebia, Lissex, France). According to the manufacturer’s instructions, 10 μl of undiluted serum were manually applied to the Hydragel agarose gels (Sebia). The subsequent steps, electrophoresis (pH 9.2, 20 W constant current at 20°C), drying, amidoblack staining, de-staining, and final drying, were carried out automatically. The use of Hydrasys densitometer and Phoresis software (Sebia) for scanning resulting profiles provided accurate relative concentrations (percentage) of individual protein zones. M-spike levels were calculated as total gamma globulins/albumin ratio (G/A).

Valtorta S., Toscani D., Chiu M., Sartori A., Coliva A., Brevi A., Taurino G., Grioni M., Ruffini L., Vacondio F., Zanardi F., Bellone M., Moresco R.M., Bussolati O, & Giuliani N. (2021). [18F](2S,4R)-4-Fluoroglutamine as a New Positron Emission Tomography Tracer in Myeloma. Frontiers in Oncology, 11, 760732.

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Quantifying Daratumumab Interference in SPEP

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A retrospective review of clinical history and laboratory results was performed with the approval of the Institutional Review Board at Memorial Sloan Kettering Cancer Center under protocol number 17-376. This analysis, represented in Figure 1, included a review of all clinical Hydrashift results and corresponding SPEP/IF results obtained from July 2018 – March 2020. We reviewed the patient’s clinical history including the start of daratumumab treatment and historical SPEP/IF results to determine the isotype and migration pattern of the patient’s original M-protein. We identified patients whose M-protein co-migrated with daratumumab on SPEP and IF and who had serial Hydrashift results. For these patients, we identified the point in their treatment at which the IF transitioned from M-protein plus daratumumab to daratumumab only (false-positive IF). The daratumumab/M-protein peak concentration was recorded at these different times.
We also identified patients who were undergoing daratumumab-based therapy and had an M-protein that was distinct from daratumumab on SPEP (Figure 2). The distinct daratumumab peak in these samples was quantified in Phoresis software (Sebia) using the perpendicular drop method.

Kirchhoff D.C., Murata K, & Thoren K.L. (2021). Use of a Daratumumab-Specific Immunofixation Assay to Assess Possible Immunotherapy Interference at a Major Cancer Center: Our Experience and Recommendations. The journal of applied laboratory medicine, 6(6), 1476-1483.

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Serum Protein Electrophoresis in Mice

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Mouse blood was periodically collected in Eppendorf by retro-orbital sampling. Semi-automated electrophoresis was performed on the Hydrasys instrument (Sebia, Lissex, France). According to the manufacturer’s instructions, 10 µL of undiluted serum were manually applied to the Hydragel agarose gels (Sebia). The subsequent steps: electrophoresis (pH 9.2, 20 W constant current at 20 °C), drying, amidoblack staining, de-staining and final drying were carried out automatically. The use of Hydrasys densitometer and Phoresis software (Sebia) for scanning resulting profiles provided accurate relative concentrations (percentage) of individual protein zones. M-spike levels were calculated as total gamma globulins/albumin ratio (G/A)^{17 (link)}.

Calcinotto A., Brevi A., Chesi M., Ferrarese R., Garcia Perez L., Grioni M., Kumar S., Garbitt V.M., Sharik M.E., Henderson K.J., Tonon G., Tomura M., Miwa Y., Esplugues E., Flavell R.A., Huber S., Canducci F., Rajkumar V.S., Bergsagel P.L, & Bellone M. (2018). Microbiota-driven interleukin-17-producing cells and eosinophils synergize to accelerate multiple myeloma progression. Nature Communications, 9, 4832.

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