The MISSION lentiviral based shRNA vector collections from Sigma Aldrich (St. Louis, MO, USA) were used for long-term silencing. Briefly, 1.6×104 cells were cultured in 96-well plates, incubated for 18–20 hours. Media was removed, hexadimethrine bromide (8 μg/ml) was added, followed by various volumes of lentiviral particles. (V-165) [TRCN0000165175] and (V-591) [TRCN0000159591] were effective KD clones. After incubation for 18–20 hours at 37°, puromycin was added and resistant colony selected and grown.
Mission lentiviral based shrna vector collections
Manufactured by Merck Group
Sourced in United States
The MISSION lentiviral based shRNA vector collections from Merck Group are a set of plasmids designed for the expression of short hairpin RNA (shRNA) sequences in target cells. These plasmids utilize a lentiviral backbone to enable efficient transduction and stable integration of the shRNA constructs into the host cell genome.
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Lab products found in correlation
2 protocols using mission lentiviral based shrna vector collections
1
Lentiviral shRNA-Mediated Gene Silencing
2
Lentiviral-based shRNA Silencing of BRD4
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The MISSION lentiviral-based shRNA vector collections from Sigma Aldrich (St. Louis, MO, USA) were used for long-term silencing of BRD4. Briefly, 1.6×104 cells were cultured in 96-well plates, incubated for 18–20 h. Hexadimethrine bromide (8 μg/ml) was added after media removal, and effective multiplicity of infection (MOI) of lentiviral particles was established. [TRCN0000199427] [TRCN0000318771] and (V-591) [TRCN0000196576] were used to identify knockdown clones. After 18–20 h incubation at 37˚C, puromycin was added at 1.0 µg/ml, and resistant clones were selected and grown.
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