A total of 5 × 106 cells were inoculated in six-well culture plates and treated with T-5224 at final concentrations of 0, 40, and 80 μM, respectively. After 24 h incubation, cells were collected and treated with Annexin V-7-AAD kit (Multi Sciences, China) and subjected to flow cytometry (Becton Dickinson, USA) to analyze apoptosis.
Annexin v 7 aad kit
Manufactured by MultiSciences Biotech
Sourced in China
The Annexin V-7-AAD kit is a laboratory tool used to detect and quantify apoptosis, a type of programmed cell death. The kit combines Annexin V, a protein that binds to a specific cellular marker, with 7-AAD, a dye that stains the DNA of cells undergoing late-stage apoptosis. The kit allows for the identification and differentiation of viable, early apoptotic, and late apoptotic/necrotic cells through flow cytometric analysis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using annexin v 7 aad kit
1
Apoptosis Analysis of T-5224 Treatment
2
Evaluating MBL-Induced Cell Apoptosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The effect of MBL on cell apoptosis was detected using the cell-cycle staining kit (MultiSciences, Hangzhou, China), according to the manufacturer’s instructions. LX-2 cells were seeded into 6-well plates overnight and then cultured in the media with the indicated concentration of MBL and/or H2O2 for 48 hours. Subsequently, the cells were collected and resuspended in 1 mL DNA staining solution and 10 μL permeabilization solution for 30 minutes in the dark. Data were acquired on a LSRII/Fortessa flow cytometer (BD Biosciences, Heidelberg, Germany) and analyzed using FlowJo software (Tree Star, Ashland, OR).
The effect of MBL on cell apoptosis was detected using the Annexin-V/7-AAD kit (MultiSciences), according to the manufacturer’s instructions. LX-2 cells were seeded into 6-well plates overnight and then cultured in the media with the indicated concentration of MBL and/or H2O2 for 48 hours. Subsequently, the cells were collected and resuspended in 100 μL Binding Buffer containing 5 μL Annexin-V and 10 μL 7-AAD for 15 minutes in the dark. Data were acquired on a LSRII/Fortessa flow cytometer (BD Biosciences) and analyzed using FlowJo software.
The effect of MBL on cell apoptosis was detected using the Annexin-V/7-AAD kit (MultiSciences), according to the manufacturer’s instructions. LX-2 cells were seeded into 6-well plates overnight and then cultured in the media with the indicated concentration of MBL and/or H2O2 for 48 hours. Subsequently, the cells were collected and resuspended in 100 μL Binding Buffer containing 5 μL Annexin-V and 10 μL 7-AAD for 15 minutes in the dark. Data were acquired on a LSRII/Fortessa flow cytometer (BD Biosciences) and analyzed using FlowJo software.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!