Ant his6 antibody

Manufactured by Abcam

The Anti-His6 antibody is a tool used to detect and purify recombinant proteins containing a hexahistidine (His6) tag. It specifically binds to the His6 tag, which is a common affinity tag added to proteins to facilitate their identification and purification.

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Lab products found in correlation

Horseradish peroxidase hrp conjugated goat anti rabbit igg, by Abcam (2 mentions) Ascpf1 cas12a antibody, by Cell Signaling Technology (2 mentions) Alkaline phosphatase, by Thermo Fisher Scientific (2 mentions) Alexa fluor 568 conjugated goat anti mouse igg, by Thermo Fisher Scientific (2 mentions) Phusion dna polymerase, by Thermo Fisher Scientific (2 mentions) Hrp conjugated anti mouse igg, by Merck Group (2 mentions) Antibiotics and dimethyl sulfoxide dmso, by Merck Group (2 mentions) Fastdigest restriction enzyme, by Thermo Fisher Scientific (1 mentions)

2 protocols using ant his6 antibody

Antibiotics and DMSO protocol

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Antibiotics and dimethyl sulfoxide (DMSO) were purchased from MilliporeSigma (St. Louis, MO, USA). Moxifloxacin stock solution was dissolved in 100% DMSO at 10 mg/mL. In all experiments, the Moxifloxacin stock was diluted in the corresponding culture medium, and controls lacking moxifloxacin were performed using an equal percentage of DMSO. FastDigest restriction enzymes, alkaline phosphatase, and DNA Phusion polymerase were purchased from ThermoFisher (Waltham, MA). The following primary antibodies were used: (i) a mouse monoclonal antibody (L2I-45) specific to the LGV L2 MOMP (48 (link)), (ii) a rabbit polyclonal anti-OmcB (kind gift from Tom Hatch, University of Tennessee), (iii) a rabbit polyclonal AsCpf1/Cas12a antibody (catalog #19984, Cell Signaling Technology), (iv) a rabbit polyclonal ant-His6 antibody (catalog #213204, Abcam), and (iv) a mouse monoclonal antibody to tubulin (catalog #T5168, MilliporeSigma). The secondary antibodies used were Alexa Fluor 568-conjugated goat anti-mouse IgG (catalog #A11004) from Invitrogen (Carlsbad, CA, USA) and horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (catalog #213204, Abcam) and HRP-conjugated anti-mouse IgG (catalog A0168, MilliporeSigma).

Shen L., Gao L., Swoboda A.R, & Ouellette S.P. (2023). Targeted repression of DNA topoisomerase I by CRISPRi reveals a critical function for it in the Chlamydia trachomatis developmental cycle. bioRxiv.

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Antibiotics and Protein Detection Methods

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibiotics and dimethyl sulfoxide (DMSO) were purchased from MilliporeSigma (St. Louis, MO, USA). Moxifloxacin stock solution was dissolved in 100% DMSO at 10 mg/mL. In all experiments, the moxifloxacin stock was diluted in the corresponding culture medium, and controls lacking moxifloxacin were performed using an equal percentage of DMSO. Digest restriction enzymes, alkaline phosphatase, and DNA Phusion polymerase were purchased from Thermo Fisher (Waltham, MA). The following primary antibodies were used to detect proteins of interests: (i) a mouse monoclonal antibody (L2I-45) specific to the LGV L2 MOMP (48 (link)); (ii) a rabbit polyclonal anti-OmcB (kind gift from Tom Hatch, University of Tennessee); (iii) a rabbit polyclonal AsCpf1/Cas12a antibody (catalog #19984, Cell Signaling Technology); (iv) a rabbit polyclonal ant-His6 antibody (catalog #213204, Abcam); and (v) a mouse monoclonal antibody to tubulin (catalog #T5168, MilliporeSigma). The secondary antibodies used were (i) Alexa Fluor 568-conjugated goat anti-mouse IgG (catalog #A11004) from Invitrogen (Carlsbad, CA, USA) and (ii) horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (catalog #213204, Abcam) and HRP-conjugated anti-mouse IgG (catalog A0168, MilliporeSigma).

Shen L., Gao L., Swoboda A.R, & Ouellette S.P. (2024). Targeted repression of topA by CRISPRi reveals a critical function for balanced DNA topoisomerase I activity in the Chlamydia trachomatis developmental cycle. mBio, 15(2), e02584-23.

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