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3 protocols using soy lecithin

1

Biocompatible Polyurethane Nanoparticles

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Polycaprolactone (PCL, Mw = 4000, CP) was purchased from Suyu plastic raw materials business department (Dongguan, China). 4,4′-diphenylmethane diisocyanate (MDI, 98%) was obtained from J&K Scientific Co., Ltd. (Beijing, China). Polyethylene glycol (PEG, Mw = 2000, CP), 1,4′-butanediol (BDO, AR), N,N-dimethylformamide (DMF, AR), soy lecithin (PC, BR), cholesterol (CH, ≥ 0.995), Tween 80 (T-80, CP), polyvinylpyrrolidone (PVP, ≥ 0.995) and anhydrous ethanol (GR) were bought from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Polyurethane (PU) was obtained from Shunte Plastic Co., Ltd. (Guangdong, China). Biaxially oriented polyethylene terephthalate (BOPET) was purchased from Yiwei Mechanical and Electrical hardware Co., Ltd. (Shanghai, China). Rosemary essential oil (REO, RG) was received from Chroma Dex Corporation (USA); 2,2-diphenyl-1-picrylhydrazyl (DPPH, ≥ 97.0%, HPLC) was acquired from Aladdin Biotechnology Co., Ltd. (Shanghai, China).
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2

Cheddar Cheese Antimicrobial Coating

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Cheddar cheese (GRANG'OR Cheddar cheese; protein 25.6%, fat 34.9%, moisture content 37.1%) was purchased from a local supermarket. Nisin (1,000 IU/ mg) was obtained from Luoyang Qihong Microbial Technology Co. Ltd. (Luoyang, China). Ammonia solution, absolute ethanol, tetraethylorthosilicate, chitosan, soy lecithin, cholesterol, trichloromethane, polyvinylpyrrolidone, and glycerol were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Listeria Chromogenic Medium was purchased from Huankai Microbial Technology Co. Ltd. (Guangzhou, China) .
Listeria monocytogenes ATCC 19115, obtained from China General Microbiological Culture Collection Center (Beijing, China), was stored in liquid paraffin wax at 4°C and cultured with shaking at 37°C for 48 h.
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3

Lemongrass Oil and Cheese Antimicrobial Evaluation

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Lemongrass oil was purchased from J.E. International (Caussols, France). Fresh hard cheese (Kerrygold Cheddar) was bought from a local supermarket. Soy lecithin, cholesterol, chloroform, and polyvinylpyrrolidone were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, P. R. China).
Listeria monocytogenes ATCC 19115 (China General Microbiological Culture Collection Center, Beijing, P. R. China) was stored in liquid paraffin wax at 4°C and cultured with shaking at 37°C for 48 h.
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