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Anti phospho perk1 2

Manufactured by Cell Signaling Technology
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The Anti-Phospho-pERK1/2 is a lab equipment product that detects phosphorylated forms of the extracellular signal-regulated kinase 1 and 2 (ERK1/2) proteins. It is designed to identify the activation state of the ERK1/2 signaling pathway.

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Lab products found in correlation

Anti phospho akt, by Cell Signaling Technology (1 mentions) Anti phospho p44 42 mapk, by Cell Signaling Technology (1 mentions) Anti phospho src tyr527, by Cell Signaling Technology (1 mentions) Anti upa, by Merck Group (1 mentions) Anti phospho pi3 kinase p85, by Cell Signaling Technology (1 mentions) P src, by Cell Signaling Technology (1 mentions) Stat3, by Cell Signaling Technology (1 mentions) E cadherin, by Cell Signaling Technology (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Protein assay, by Bio-Rad (1 mentions) β cadherin, by Cell Signaling Technology (1 mentions) P creb, by Cell Signaling Technology (1 mentions) Prsk2, by Cell Signaling Technology (1 mentions) Fascin, by Cell Signaling Technology (1 mentions) P stat3 y705, by Cell Signaling Technology (1 mentions) P fak, by Cell Signaling Technology (1 mentions) Snail, by Cell Signaling Technology (1 mentions) Vimentin, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Anti-phospho-p44/42 MAPK (pERK1/2), (6 citations) Phospho-pERK1/2 (4 citations) Anti-phospho-p44/42 (pERK1/2; (2 citations)

2 protocols using anti phospho perk1 2

1

Quantification of Protein Signaling Pathways

Cited 1 time
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Western blot was performed according to a previously described method [32 (link)] with antibodies for anti-human KLF17 (1:200, Santa Cruz, CA; 1:200, Sigma, CA), anti-uPA (1:200, Sigma, CA), anti-Phospho-p38/MAPK, anti-Phospho-pERK1/2, anti-Phospho-pSAPK/JNK, anti-Phospho-Akt, anti-Phospho-p44/42/MAPK, anti- Phospho-PI3-Kinasep85 and anti-Phospho-Src (Tyr527) (Cell Signaling Tech., Danvers, MA). Anti-GAPDH (Bioworld Tech, Inc., Nanjing, China) was used to detect the internal control.
Cai X.D., Che L., Lin J.X., Huang S., Li J., Liu X.Y., Pan X.F., Wang Q.Q., Chen L., Lin M.J., Huang Z.H., Ma H.M., Wu Y., Liu S.M, & Zhou Y.B. (2017). Krüppel-like factor 17 inhibits urokinase plasminogen activator gene expression to suppress cell invasion through the Src/p38/MAPK signaling pathway in human lung adenocarcinoma. Oncotarget, 8(24), 38743-38754.
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2

Immunoblot Analysis of Signaling Pathways in Tumor Xenografts

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Protein concentrations of the total cellular protein extracts from tumor xenografts and cell line lysates were determined using the Bio-Rad protein assay (Bio-Rad, Hercules, CA, USA). Total protein (50 μg per sample) from either tumor xenograft or cell line lysates was electrophorectically separated on 10% SDS polyacrylamide gels and electro-blotted onto a Hybond membrane. After incubation with 5% nonfat milk in Tris-buffered saline containing 0.1% Tween-20, the blots were probed with anti-phospho p-Erk1/2 (Thr202/Tyr204), p-Creb (Ser133), p-Rsk2 (Ser386), p-Src (Tyr416), p-Fak (Tyr576/577), p-Pax (y118), p-Pkcα (s657), and p-Stat3 (y705), polyclonal rabbit antibodies (Cell Signaling Technology Inc., Davners, MA). The same blots were subsequently stripped and re-probed with anti-total (t) Her2, Her3, Erk1/2, cSrc, Creb, Fax, Pax, Pkcα, Rsk2, Stat3, Fascin, E-Cadherin, β-Cadherin, Vimentin, Snail, and β-Actin antibodies (Cell Signaling Technology Inc.).
Mao L., Summers W., Xiang S., Yuan L., Dauchy R.T., Reynolds A., Wren-Dail M.A., Pointer D., Frasch T., Blask D.E, & Hill S.M. (2016). Melatonin Represses Metastasis in Her2-postive Human Breast Cancer Cells by Suppressing RSK2 Expression. Molecular cancer research : MCR, 14(11), 1159-1169.
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