Cytotoxicity bioassay kit

The cell viability assay was performed as previously described [26 (link)]. Briefly, Vero cells were grown in 96-well plates and treated with different concentrations of OESA and OESY extracts (0.05 mg/mL, 0.1 mg/mL, 0.2 mg/mL, 0.4 mg/mL, 0.8 mg/mL, and 1 mg/mL) for 72 h. The cell viability was determined with a cytotoxicity bioassay kit (Lonza Group Ltd., Basel, Switzerland) according to the manufacturer’s instructions. The GloMax^® Multi Microplate Luminometer (Promega Corporation, 2800 Woods Hollow Road, Madison, WI, USA) in combination with the ViaLight™ plus cell proliferation and cytotoxicity bioassay kit was used to detect the emitted light intensity related to ATP degradation. The measured luminescence value was converted into the cell proliferation index (%) as previously reported [19 (link)].

Vero cells were grown in wells of 96-well plates and treated with three different concentrations of almond extracts (0.4 mg/mL, 0.2 mg/mL and 0.1 mg/mL). The cell viability was determined with a cytotoxicity bioassay kit (Lonza Group Ltd., Basel, Switzerland) according to the manufacturer’s instructions. The GloMax^® Multi Microplate Luminometer (Promega Corporation, 2800 Woods Hollow Road, Madison, WI, USA) in combination with the ViaLight^™ plus cell proliferation and cytotoxicity bioassay kit quantified the emitted light intensity related to ATP degradation. The measured luminescence value was converted to the cell proliferation index (%) according to the following Equation (1): $\mathrm{Cell}\mathrm{viability}\%=(\frac{A-B}{C-B})\%$
where A denotes the average of treated sample, B represents background luminescence and C represents the average of untreated samples.