Immunofluorescence assay was carried out following a previously published protocol.10 Anti‐GFAP Ab (1:2000, M0761; Dako), rabbit monoclonal anti‐GSN Ab (1:3000 dilution; EPR1942, ab109014; Abcam), and Alexa Fluor 568 phalloidin (1:1000 dilution; Thermo Fisher Scientific) were used.
Ab109014
Manufactured by Abcam
Ab109014 is a rabbit monoclonal antibody that recognizes the human Vimentin protein. Vimentin is a type III intermediate filament protein that is commonly used as a marker for mesenchymal cells. The antibody is suitable for use in various immunoassay applications, such as Western blotting and immunohistochemistry.
Automatically generated - may contain errors
Lab products found in correlation
M0761, by Agilent Technologies (1 mentions)
Alexa fluor 568 phalloidin, by Thermo Fisher Scientific (1 mentions)
Epr1942, by Abcam (1 mentions)
Ripa lysis buffer, by Beyotime (1 mentions)
Ab26350, by Abcam (1 mentions)
Ab184787, by Abcam (1 mentions)
Enhanced chemiluminescence western blot detection system, by Bio-Rad (1 mentions)
3 protocols using ab109014
1
Immunofluorescence Assay for GFAP, GSN, and F-actin
2
Protein Extraction and Western Blot Analysis for Tumor Xenograft Studies
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tumour tissues of subcutaneous tumour xenograft or cells were lysed on ice and total protein was extracted using RIPA lysis buffer (Beyotime Institute of Biotechnology). The protein concentration was measured using Coomassie Brilliant Blue (APPLYGEN A1011). Equal amounts of protein lysates (30 μg/lane) was subjected to electrophoresis on 10% SDS‐polyacrylamide gels and then transferred to PVDF membrane (cat. no. IPVH00010; EMD Millipore). The membranes were blocked with 5% milk (BD Biosciences) for 1 h and then incubated with corresponding primary antibody overnight at 4°C.The results were analysed using an enhanced chemi‐luminescence western blot detection system (Bio‐Rad Laboratories). In this study, the antibodies used were as followed: Rabbit anti‐gelsolin polyclonal antibody (1:1000; ab109014; abcam) Mouse anti‐γH2AX monoclonal antibody (1:1000; ab26350; abcam) Rabbit anti‐Caspase‐3 Monoclonal antibody (1:1000; ab184787; abcam).
3
Western Blot Analysis of GSN and GSK3β
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Western blot analysis was undertaken as previously described.11 The PVDF membranes were incubated with a primary rabbit monoclonal anti‐GSN Ab (1:25 000, EPR1942, ab109014; Abcam), rabbit anti‐p‐GSK3βSer9 Ab (9336s, 1:1000; Cell Signaling Technology), mouse anti‐p‐GSK3βTyr216 Ab (612 313, 1:1000; BD Biosciences), and mouse anti‐GSK3β Ab (610 202, 1:1000, BD Biosciences).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!