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Sodium citrate buffer ph 6

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Sodium citrate buffer pH 6.0 is a buffer solution primarily used to maintain a specific pH environment in various laboratory applications. It is composed of sodium citrate and citric acid, formulated to provide a stable pH of 6.0. This buffer is commonly used to facilitate biochemical reactions, cell culture, or other processes that require a slightly acidic pH environment.

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Lab products found in correlation

Antibody diluent background reducing solution, by Agilent Technologies (2 mentions) Envision plus dual labeled polymer kit, by Agilent Technologies (2 mentions) Anti ps744 748 pkd antibody, by Abcam (2 mentions) Protein block serum free solution, by Agilent Technologies (2 mentions)

Spelling variants of this product

Citrate buffer (123 citations) Citrate buffer pH 6 (33 citations) Citrate pH 6 (3 citations) Sodium‐citrate buffer (3 citations) Sodium citrate (3 citations)

2 protocols using sodium citrate buffer ph 6

1

Immunohistochemical Analysis of PKD Proteins

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Slides were de-paraffinized (1 hr, 60° C), de-waxed in xylene (5 times, 4 min) and gradually re-hydrated with ethanol (100%, 95%, 75%, each 2 times, 3 min). Re-hydrated samples were rinsed in water and subjected to antigen retrieval in 10 mM sodium citrate buffer pH 6.0 (DAKO, Carpinteria, CA). Slides were treated with 3% H2O2 (5 min) to reduce endogenous peroxidase activity, washed with PBS containing 0.5% Tween 20, and blocked with protein block serum free solution (DAKO) for 5 min at RT. Samples were stained with H&E, alcian blue, anti-claudin-18 antibody (1:1000), anti-PKD1 antibody (1:100), anti-PKD2 antibody (1:200), anti-PKD3 antibody (1:200), or anti-pS744/748-PKD antibody (Abcam) at a dilution of 1:50 in Antibody Diluent Background Reducing Solution (DAKO) and visualized using the Envision Plus Dual Labeled Polymer Kit (DAKO) according to the manufacturer’s instructions. Images were captured using the ScanScope XT scanner and ImageScope software (Aperio, Vista, CA).
Liou G.Y., Döppler H., Braun U.B., Panayiotou R., Buzhardt M.S., Radisky D.C., Crawford H.C., Fields A.P., Murray N.R., Wang Q.J., Leitges M, & Storz P. (2015). Protein kinase D1 drives pancreatic acinar cell reprogramming and progression to intraepithelial neoplasia. Nature communications, 6, 6200.
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2

Immunohistochemical Analysis of PKD Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Slides were de-paraffinized (1 hr, 60° C), de-waxed in xylene (5 times, 4 min) and gradually re-hydrated with ethanol (100%, 95%, 75%, each 2 times, 3 min). Re-hydrated samples were rinsed in water and subjected to antigen retrieval in 10 mM sodium citrate buffer pH 6.0 (DAKO, Carpinteria, CA). Slides were treated with 3% H2O2 (5 min) to reduce endogenous peroxidase activity, washed with PBS containing 0.5% Tween 20, and blocked with protein block serum free solution (DAKO) for 5 min at RT. Samples were stained with H&E, alcian blue, anti-claudin-18 antibody (1:1000), anti-PKD1 antibody (1:100), anti-PKD2 antibody (1:200), anti-PKD3 antibody (1:200), or anti-pS744/748-PKD antibody (Abcam) at a dilution of 1:50 in Antibody Diluent Background Reducing Solution (DAKO) and visualized using the Envision Plus Dual Labeled Polymer Kit (DAKO) according to the manufacturer’s instructions. Images were captured using the ScanScope XT scanner and ImageScope software (Aperio, Vista, CA).
Liou G.Y., Döppler H., Braun U.B., Panayiotou R., Buzhardt M.S., Radisky D.C., Crawford H.C., Fields A.P., Murray N.R., Wang Q.J., Leitges M, & Storz P. (2015). Protein kinase D1 drives pancreatic acinar cell reprogramming and progression to intraepithelial neoplasia. Nature communications, 6, 6200.
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