Z val ala dl asp ome fluoromethylketone z vad

Manufactured by Bachem

Sourced in Germany

Z-Val-Ala-DL-Asp(OMe)-fluoromethylketone (z-VAD) is a synthetic compound that functions as a broad-spectrum caspase inhibitor. It is commonly used in biochemical research and experimentation.

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Lab products found in correlation

Staurosporine, by Alexis Biochemicals (1 mentions) Gemcitabine, by Fresenius (1 mentions) Cisplatin, by Teva Pharmaceuticals (1 mentions) Bafilomycin a1 bafa1, by Merck Group (1 mentions) Lysotracker red dnd 99, by Thermo Fisher Scientific (1 mentions) Abt 737, by Santa Cruz Biotechnology (1 mentions) Gossypol, by Bio-Techne (1 mentions) 3 methyladenin 3 ma, by Merck Group (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Lps escherichia coli o111 b4, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Dmem medium, by Thermo Fisher Scientific (1 mentions) Necrostatin 1 nec 1, by Selleck Chemicals (1 mentions) Birinapant, by Apexbio (1 mentions) Celltiter glo 2, by Promega (1 mentions) Deep blue cell viability kit, by BioLegend (1 mentions) Synergy h1 hybrid reader, by Agilent Technologies (1 mentions)

3 protocols using z val ala dl asp ome fluoromethylketone z vad

Apoptosis and Autophagy Inhibitor Assay

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The pan Bcl-2 inhibitor (−)-gossypol (>98% purity) was acquired from Tocris (Bristol, United Kingdom). Autophagy inhibitors Bafilomycin A1 (BafA1), and 3-Methyladenin (3-MA) were obtained from Sigma-Aldrich (Taufkirchen, Germany). The pan-caspase inhibitor z-Val-Ala-DL-Asp(OMe)-fluoromethylketone (z-VAD) was purchased from Bachem (Weil am Rhein, Germany). The Mcl-1 sparing Bcl-2 inhibitor ABT-737 was from Santa Cruz Biotechnology (Heidelberg, Germany) and the inductor of apoptotic cell death staurosporine (STS) was from Alexis Biochemicals (by ENZO Life Sciences, Lörrach, Germany). Chemotherapeutic gemcitabine was from Fresenius-Kabi (Bad Homburg, Germany) and chemotherapeutic cisplatin from Teva (Ulm, Germany). Lysotracker Red DND-99 was obtained from Invitrogen (Karlsruhe, Germany). All other chemicals were used in analytic grade purity from Sigma-Aldrich.

Mani J., Vallo S., Rakel S., Antonietti P., Gessler F., Blaheta R., Bartsch G., Michaelis M., Cinatl J., Haferkamp A, & Kögel D. (2015). Chemoresistance is associated with increased cytoprotective autophagy and diminished apoptosis in bladder cancer cells treated with the BH3 mimetic (−)-Gossypol (AT-101). BMC Cancer, 15, 224.

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Apoptosis Induction in RAW 264.7 Cells

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The RAW 264.7 cells and 293T cells were cultured in the DMEM medium (Gibco) supplemented with 10% FBS (Gibco), streptomycin (0.1 mg/mL) and penicillin (0.06 mg/mL) (Gibco). To induce apoptosis, RAW 264.7 cells at 40% confluence were incubated in DMEM (10% FBS) containing 2 μg/mL lipopolysaccharide (2 μg/mL, LPS; Escherichia coli O111:B4, Sigma-Aldrich, >500,000 EU/mg) and murine IFN-γ in 0.1 % DMSO (50 U/mL, Peprotech, >98%), or LPS and IFN-γ plus TZ in 0.1 % DMSO (10 μM) for 18 to 30 hours. For H₂O₂ treatment, the final concentration of H₂O₂ was 1 mM. For geldanamycin (GA, purchased from Biovision, >99%) treatment, the concentration was 10 nM. Z-Val-Ala-DL-Asp (OMe)-fluoromethylketone (Z-vad, Bachem) was used at the final concentration of 100 μ;M.

Chen X., Zhao C., Li X., Wang T., Li Y., Cao C., Ding Y., Dong M., Finci L., Wang J.H., Li X, & Liu L. (2014). Terazosin activated Pgk1 and Hsp90 to promote stress resistance. Nature chemical biology, 11(1), 19-25.

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Assessing Cell Death Pathways

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Cells were plated at ~80-90% confluency in a 96-well plate the day before. Different concentrations of the inhibitors were required to induce RCD in different cell lines. hTERTs and A549s were pretreated with 10 μM of the SMAC-mimetic, Birinapant (ApexBio: A4219), 100 μM of the pan-caspase inhibitor, Z-Val-Ala-DL-Asp(OMe)-fluoromethylketone (zVAD) (Bachem 4027403), and/or 10 μM of the RIPK1 inhibitor, Necrostatin-1 (Nec-1) (Selleck Chemicals S8037) for 30 min before a 24 hr stimulation with human or murine TNFα. Jurkats and mouse-ear fibroblasts were treated with 1 μM Birinapant, 25 μM zVAD, and/or 5 μM MRT67307. Viability was measured with the Deep Blue Cell Viability Kit (BioLegend 424702) or CellTiter-Glo 2.0 (Promega G9241) on a Synergy H1 Hybrid Reader (BioTEK).

Taft J., Markson M., Legarda D., Patel R., Chan M., Malle L., Richardson A., Gruber C., Martín-Fernández M., Mancini G.M., van Laar J.A., van Pelt P., Buta S., Wokke B.H., Sabli I.K., Sancho-Shimizu V., Chavan P.P., Schnappauf O., Khubchandani R., Cüceoğlu M.K., Özen S., Kastner D., Ting A.T., Aksentijevich I., Hollink I.H, & Bogunovic D. (2021). Human TBK1 Deficiency Leads to Autoinflammation Driven by TNF-Induced Cell Death. Cell, 184(17), 4447-4463.e20.

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