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Hbd mutant aav dj 8 serotype

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The HBD mutant AAV-DJ/8 serotype is a laboratory equipment product designed for research purposes. It is a modified adeno-associated virus (AAV) vector that can be used for various applications in cell and gene therapy research. The core function of this product is to serve as a delivery vehicle for genetic material into target cells. No further details or interpretations are provided.

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Lab products found in correlation

Paav flex tdtomato, by Addgene (2 mentions)

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AAV-DJ (6 citations)

2 protocols using hbd mutant aav dj 8 serotype

1

Endothelial-Specific PICALM Rescue in AD Mice

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Endothelial specific rescue of PICALM was achieved by adeno-associated viral delivery of a Cre-dependent expression cassette (Flex-Picalm) to 5 month old APPsw/0; Picalm+/−; Tie2-Cre mice. The strategy was first validated with AAV-Flex-tdTomato virus (100 nl, 5x1012 GC/ml) and AAV-synapsin-GFP virus (20 nl, 5x1012 GC/ml) that were injected simultaneously into the hippocampus. AAV-Flex-Picalm construct was generated by cloning the coding region of mouse Picalm gene into the pAAV-FLEX-tdTomato (Addgene, Plasmid #28306) to replace the tdTomato. AAV-Flex-Picalm viruses were packaged by Penn Vector Core (6x1012 GC/ml) and 100 nl was used for each injection. The HBD mutant AAV-DJ/8 serotype (Cell Biolabs) was chosen for its high in vivo transduction efficiency and vascular tropism54 .
Zhao Z., Sagare A.P., Ma Q., Halliday M.R., Kong P., Kisler K., Winkler E.A., Ramanathan A., Kanekiyo T., Bu G., Owens N.C., Rege S.V., Si G., Ahuja A., Zhu D., Miller C.A., Schneider J.A., Maeda M., Maeda T., Sugawara T., Ichida J.K, & Zlokovic B.V. (2015). Central role for PICALM in amyloid–β blood–brain barrier transcytosis and clearance. Nature neuroscience, 18(7), 978-987.
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2

Endothelial-Specific PICALM Rescue in AD Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Endothelial specific rescue of PICALM was achieved by adeno-associated viral delivery of a Cre-dependent expression cassette (Flex-Picalm) to 5 month old APPsw/0; Picalm+/−; Tie2-Cre mice. The strategy was first validated with AAV-Flex-tdTomato virus (100 nl, 5x1012 GC/ml) and AAV-synapsin-GFP virus (20 nl, 5x1012 GC/ml) that were injected simultaneously into the hippocampus. AAV-Flex-Picalm construct was generated by cloning the coding region of mouse Picalm gene into the pAAV-FLEX-tdTomato (Addgene, Plasmid #28306) to replace the tdTomato. AAV-Flex-Picalm viruses were packaged by Penn Vector Core (6x1012 GC/ml) and 100 nl was used for each injection. The HBD mutant AAV-DJ/8 serotype (Cell Biolabs) was chosen for its high in vivo transduction efficiency and vascular tropism54 .
Zhao Z., Sagare A.P., Ma Q., Halliday M.R., Kong P., Kisler K., Winkler E.A., Ramanathan A., Kanekiyo T., Bu G., Owens N.C., Rege S.V., Si G., Ahuja A., Zhu D., Miller C.A., Schneider J.A., Maeda M., Maeda T., Sugawara T., Ichida J.K, & Zlokovic B.V. (2015). Central role for PICALM in amyloid–β blood–brain barrier transcytosis and clearance. Nature neuroscience, 18(7), 978-987.
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