Hsp10

Manufactured by Enzo Life Sciences

HSP10 is a molecular chaperone protein that assists in the folding and unfolding of other proteins. It is involved in the cellular stress response and plays a role in protein homeostasis.

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Lab products found in correlation

Hsp70, by Enzo Life Sciences (2 mentions) Trpv1, by Novus Biologicals (1 mentions) Hsp40, by Enzo Life Sciences (1 mentions) Stim1, by OriGene (1 mentions) Enhanced chemiluminescence reagent, by Cytiva (1 mentions) Horseradish peroxidase conjugated secondary antibody, by Cytiva (1 mentions) Phospho erk, by Cell Signaling Technology (1 mentions) Orai1, by Merck Group (1 mentions) β actin, by Santa Cruz Biotechnology (1 mentions) Catalase, by Abcam (1 mentions) Parkin, by Abcam (1 mentions) Total oxphos, by Abcam (1 mentions) Nrf 1, by Rockland Immunochemicals (1 mentions) Pink1, by Abcam (1 mentions) Cytochrome c, by Santa Cruz Biotechnology (1 mentions)

2 protocols using hsp10

Evaluation of Key Signaling Proteins

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Total cell lysates (100 μg) were analyzed using SDS-PAGE on a 12% gel. After electro-blotting to a nitrocellulose membrane, membranes were blocked with 1% BSA for 1 h at room temperature. Membranes were washed with 0.1% TBST three times and then incubated with primary antibodies overnight at 4°C. Antibodies against Orai1(Merck Millipore), STIM1(OriGene), TRPV1 (Novus Biologicals), phospho-ERK (Cell Signaling Technology), ERK (Cell Signaling Technology), phosphor-JNK (BD Transduction Laboratories^™), JNK (BD Transduction Laboratories^™), phosphor-p38 (BD Transduction Laboratories^™), p38 (BD Transduction Laboratories^™), HSP10 (Enzo Life Sciences), HSP40 (Enzo Life Sciences), HSP70 (Enzo Life Sciences), HSP90 (Calbiochem, Merck Millipore), and β-actin (Santa Cruz) were utilized as the primary antibodies. The membranes were then treated with horseradish peroxidase-conjugated secondary antibodies (Amersham Biosciences). Immunoreactive proteins were visualized using enhanced chemiluminescence reagents (Amersham Biosciences).

Wu C.Y., Hsu W.L., Wang C.H., Liang J.L., Tsai M.H., Yen C.J., Li H.W., Chiu S.J., Chang C.H., Huang Y.B., Lin M.W, & Yoshioka T. (2016). A Novel Strategy for TNF-Alpha Production by 2-APB Induced Downregulated SOCE and Upregulated HSP70 in O. tsutsugamushi-Infected Human Macrophages. PLoS ONE, 11(7), e0159299.

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Mitochondrial Protein Abundance Analysis

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The abundance of SS and IMF mitochondrial proteins was determined via western blot as previously described (Kavazis et al., 2009a ; Smuder et al., 2012 (link)). Specifically, SS and IMF mitochondrial proteins were probed for: cytochrome c, AIF, SOD1, SOD2 (Santa Cruz Biotechnology, Dallas, TX), OPA1, DRP1 (BD, Franklin Lakes, NJ), total OXPHOS, GPX1, catalase, p62, Parkin, PINK1 (Abcam, Cambridge, MA), Tfam (Calbiochem, Darmstadt, Germany), Nrf1 (Rockland Immunochemicals, Limerick, PA), pUB (Boston Biochem, Cambridge, MA), Fis1, HSP70, HSP10 (Enzo Life Sciences, Farmingdale, NY), Mfn2 and LON (Sigma, St. Louis, MO). VDAC (Santa Cruz Biotechnology) was used as a mitochondrial marker to control for protein loading and transfer differences.

Kavazis A.N., Morton A.B., Hall S.E, & Smuder A.J. (2016). Effects of doxorubicin on cardiac muscle subsarcolemmal and intermyofibrillar mitochondria. Mitochondrion, 34, 9-19.

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