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Axioimager fluorescence microscope

Manufactured by Hamamatsu Photonics
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The AxioImager fluorescence microscope is a high-performance imaging system designed for advanced fluorescence microscopy applications. It provides precise and accurate imaging capabilities, enabling researchers to capture detailed images of fluorescently labeled samples.

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Lab products found in correlation

Donkey anti goat secondary antibody, by Thermo Fisher Scientific (2 mentions) Chicken anti gfp, by Thermo Fisher Scientific (2 mentions) Hoechst 33258, by Merck Group (2 mentions) Rabbit anti mcherry, by Thermo Fisher Scientific (2 mentions) Orca 03 camera, by Hamamatsu Photonics (2 mentions) Goat anti sox9, by R&D Systems (2 mentions)

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Fluorescence microscope (11 citations) AxioImager microscope (4 citations)

2 protocols using axioimager fluorescence microscope

1

Embryonic Limb Development Immunostaining

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Mouse embryonic limbs at E10.5, E11.5 or E12.5 were dissected in cold PBS and fixed in 4% PFA for 2–3 hours. Following incubation in a sucrose gradient and embedding in a 1:1 mixture of 30% Sucrose and OCT, sagittal 10 μm frozen sections were cut using a cryostat. Cryosections were incubated overnight with the following primary antibodies: chicken anti-GFP (1:500, Thermo Fisher Scientific, A10262), rabbit anti-mCherry (1:1,000, Thermo Fisher Scientific, PA5-34974) and goat anti-Sox9 (1:500, R&D Systems, AF3075). Goat-anti chicken, goat anti-rabbit and donkey anti-goat secondary antibodies conjugated to Alexa Fluor 488, 568, 594 or 647 (1:1,000, Thermo Fisher Scientific) were used for detection. Hoechst 33258 (Sigma) was utilized to counterstain nuclei. Fluorescent images were acquired using a Zeiss AxioImager fluorescence microscope in combination with a Hamamatsu Orca-03 camera. Brightness and contrast were adjusted uniformly using Photoshop CS5.
Osterwalder M., Barozzi I., Tissières V., Fukuda-Yuzawa Y., Mannion B.J., Afzal S.Y., Lee E.A., Zhu Y., Plajzer-Frick I., Pickle C.S., Kato M., Garvin T.H., Pham Q.T., Harrington A.N., Akiyama J.A., Afzal V., Lopez-Rios J., Dickel D.E., Visel A, & Pennacchio L.A. (2018). Enhancer Redundancy Allows for Phenotypic Robustness in Mammalian Development. Nature, 554(7691), 239-243.
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2

Embryonic Limb Development Immunostaining

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse embryonic limbs at E10.5, E11.5 or E12.5 were dissected in cold PBS and fixed in 4% PFA for 2–3 hours. Following incubation in a sucrose gradient and embedding in a 1:1 mixture of 30% Sucrose and OCT, sagittal 10 μm frozen sections were cut using a cryostat. Cryosections were incubated overnight with the following primary antibodies: chicken anti-GFP (1:500, Thermo Fisher Scientific, A10262), rabbit anti-mCherry (1:1,000, Thermo Fisher Scientific, PA5-34974) and goat anti-Sox9 (1:500, R&D Systems, AF3075). Goat-anti chicken, goat anti-rabbit and donkey anti-goat secondary antibodies conjugated to Alexa Fluor 488, 568, 594 or 647 (1:1,000, Thermo Fisher Scientific) were used for detection. Hoechst 33258 (Sigma) was utilized to counterstain nuclei. Fluorescent images were acquired using a Zeiss AxioImager fluorescence microscope in combination with a Hamamatsu Orca-03 camera. Brightness and contrast were adjusted uniformly using Photoshop CS5.
Osterwalder M., Barozzi I., Tissières V., Fukuda-Yuzawa Y., Mannion B.J., Afzal S.Y., Lee E.A., Zhu Y., Plajzer-Frick I., Pickle C.S., Kato M., Garvin T.H., Pham Q.T., Harrington A.N., Akiyama J.A., Afzal V., Lopez-Rios J., Dickel D.E., Visel A, & Pennacchio L.A. (2018). Enhancer Redundancy Allows for Phenotypic Robustness in Mammalian Development. Nature, 554(7691), 239-243.
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