The femoral condyles were excised and fixed in a 10% buffered neutral formalin solution for 1 week at room temperature at weeks 4, 24 and 48. Following decalcification, the samples were cut in half longitudinally and embedded in paraffin wax. Central sections (5 µm thick) were cut from the femoral condyle with a Leitz 1512 microtome (Leica Microsystems GmbH, Wetzlar, Germany) and stained at room temperature for 10 min with hematoxylin and eosin (H&E) for histological examination. Microscopic images were captured using a light microscope (dotSlide Virtual Slide System; Olympus Corporation, Tokyo, Japan) at ×40, ×100 and ×200 magnification.
Dotslide virtual slide system
Manufactured by Olympus
Sourced in Japan
The DotSlide Virtual Slide System is a digital microscopy solution designed for versatile imaging applications. It enables the digitization and analysis of various types of microscopic samples, facilitating efficient data management and collaboration.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using dotslide virtual slide system
1
Histological Analysis of Femoral Condyles
2
Paraffin Embedding and Histology
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PFA-fixed samples were processed through graded ethanol (50–100%) and histoclear (100%) prior to embedding in paraffin wax. Sequential sections were cut at 7 µm on the microtome and mounted on glass slides for histological and immunohistochemical staining. Images were captured with Olympus dotSlide virtual slide system (Olympus Microscopy, Southend-on-Sea, UK).
3
Morphometric Analysis of Intestinal Tissues
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As previously described [26 (link)], the segments of the jejunum and ileum tissues were fixed in 4% formaldehyde and embedded in paraffin. Sections of 5 μm thickness were stained with hematoxylin and eosin (H&E). Photomicrographs were acquired using an Olympus IX83P2ZF microscope (Olympus, Tokyo, Japan). The morphometric analysis was performed on 10 randomly-selected, well-oriented villi and crypts per piglet. A computerized microscope-based image analyzer (Olympus dotslide Virtual Slide System, Tokyo, Japan) was used to determine the height of villus (from the tip of the villus to the villus-crypt junction) and crypt depth (from the crypt-villus junction to the base of the crypt).
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