720 es inductively coupled plasma optical emission spectrometer

Pigs were sacrificed by exsanguination under deep anesthesia at the end of the study period (12 weeks). Two pigs were prematurely euthanized. One pig (19S018) was euthanized at 2 weeks owing to the presence of pulmonary embolism after the autologous clot deployment, and one pig (20S001) was prematurely euthanized at 10 weeks owing to a laboratory shutdown due to the COVID-19 pandemic. All animals were necropsied and the IVC, pulmonary arteries, lungs, heart, liver, kidneys, and spleen were collected. Harvested organs were weighed and placed in a 50 mL screwcap polypropylene sample tube prior to digestion with nitric acid. After digestion, an aliquot of 1 mL from each sample was diluted to 15 mL with a 5% solution of nitric acid. The samples were then passed through a 0.2 μm filter and the bismuth content was quantified with Varian 720-ES inductively coupled plasma optical emission spectrometer.
Histological evaluation was done by dividing the IVC into 2 portions where the upper portion was evaluated for incorporation within the vessel wall, integrity, and for the degree of filter resorption, while the lower portion was assessed for histological changes with hematoxylin and eosin staining. Polarized light microscopy was used to confirm the presence of PPDO suture material in the IVC and lungs.