Citrullinated histone H3 (H3Cit) was quantified with a slight modification of the ELISA previously described by Thalin et al. (19 (link)) by using Cell Death detection kit without streptavidin-precoated wells. The optical densities (ODs) were measured at a wavelength of 450 nm with a reference correction wavelength at 620 nm using a microplate photometer (Infinite® 200 PRO NanoQuant Multimode Microplate Reader, Tecan).
Infinite 200 pro nanoquant multimode microplate reader
Manufactured by Tecan
Sourced in Switzerland
The Infinite® 200 PRO NanoQuant Multimode Microplate Reader is a laboratory instrument designed for precise and accurate measurements of sample concentrations in microplates. It is capable of detecting and quantifying various analytes, including proteins, nucleic acids, and small molecules.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using infinite 200 pro nanoquant multimode microplate reader
1
Quantification of Citrullinated Histone H3
2
Quantifying Circulating Cell-free DNA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The Quant-it™ PicoGreen assay kit (Invitrogen, San Diego, CA, USA) was used to quantify circulating cell-free double-strand DNA according to manufacturer’s instructions. Fluorescence intensity was measured using a microplate photometer (Infinite® 200 PRO NanoQuant Multimode Microplate Reader, Tecan; 480 nm excitation wavelength/523 nm emission wavelength).
3
MTT Assay for Cell Viability
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell viability was estimated using MTT assay. Briefly, cells were seeded into 96-well plates at a density of 1 × 104 cells/mL. On the following day, cells were incubated with drugs diluted in cell culture medium for an appropriate time. Cells were then incubated with MTT (0.5 mg/mL) for additional 3 h, and the medium was replaced with DMSO (100 μL/well) to dissolve the formazan crystals. The absorbance at 570 nm was measured using Infinite® 200 PRO NanoQuant Multimode Microplate Reader (Tecan Trading AG, Switzerland). Assays were repeated at least three times in quintuplicate.
4
MTT Assay for Cell Viability
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MTT assay was used to detect cell viability. The cells were inoculated in 96-well plates at a density of 1 × 104 cells/mL. On the second day, after the cells were completely adherent, the drug was diluted to a suitable concentration using the medium, and then it was used to culture the cells for a specified time. After that, 20 uL MTT (0.5 mg/mL) was added to each well, and the culture was continued for 3 h. Finally, the medium was discarded and DMSO (100 μL/well) was added to dissolve the formazan crystal. The absorbance at 570 nm was measured using Infinite® 200 PRO Nano Quant Multimode Microplate Reader (Tecan Trading AG, Männedorf, Switzerland). Each group had 5 replicates. The detection was repeated at least 3 times.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!