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Lentiviral vectors and plasmids expressing il 6r shrna

Manufactured by GenePharma
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Sourced in China, United States

Lentiviral vectors and plasmids expressing IL-6R-shRNA are genetic tools designed for gene silencing experiments. They contain a sequence encoding a short hairpin RNA (shRNA) targeting the interleukin-6 receptor (IL-6R) gene. These tools can be used to investigate the role of IL-6R in cellular processes.

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Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) β actin, by Merck Group (1 mentions)

Spelling variants of this product

Lentiviral vector (46 citations) Lentiviral shRNAs (6 citations) ShRNA lentiviral vectors (4 citations) Lentiviral vectors expressing (3 citations) Lentiviral plasmids (2 citations) ShRNA plasmid (2 citations) ShRNA vectors (2 citations)

2 protocols using lentiviral vectors and plasmids expressing il 6r shrna

1

Lentiviral Silencing of IL-6R in LCSCs

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Lentiviral vectors and plasmids expressing IL-6R-shRNA were purchased from Shanghai Gene-Pharma Co. (Shanghai, China). The shRNA was transfected into LCSCs using LipofectamineTM 2000 (Life Technologies) according to the manufacturer’s instructions. Transfection efficiency was determined by RT-qPCR and Western blotting after 24 hours.
Li Y., Chen G., Han Z., Cheng H., Qiao L, & Li Y. (2020). IL-6/STAT3 Signaling Contributes to Sorafenib Resistance in Hepatocellular Carcinoma Through Targeting Cancer Stem Cells. OncoTargets and therapy, 13, 9721-9730.
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2

Antibody Sourcing and Lentiviral Knockdown

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All antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). β-Actin was purchased from Sigma-Aldrich (St Louis, MO, USA), and lentiviral vectors and plasmids expressing IL-6R-shRNA were purchased from Shanghai Gene-Pharma Co.
Li Y., Chen G., Han Z., Cheng H., Qiao L, & Li Y. (2020). IL-6/STAT3 Signaling Contributes to Sorafenib Resistance in Hepatocellular Carcinoma Through Targeting Cancer Stem Cells. OncoTargets and therapy, 13, 9721-9730.
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