Cc7650 sem carbon coater unit

The previously prepared material sample discs were selected for the analysis (n = 5 per material). After the incubation period, the set material discs were coated with carbon under a CC7650 SEM Carbon Coater Unit (Quorum Technologies Ltd.). The superficial element distribution of the coated discs was then individually examined in a scanning electron microscopy (SEM) unit (Jeol 6100 EDAX; Jeol Inc.) attached to an energy dispersive spectroscopy (EDS) system (INCA 350 EDS; Oxford Instruments) for the elemental analysis.

The surface and morphology analyses of the different materials were performed using SEM-coupled energy-dispersive spectroscopy (SEM–EDS, JSM-610LV, JEOL, Tokyo, Japan). Specimen disks of each material (n = 9) were prepared, and after setting time at 37 °C and 95% humidity, the disks underwent a carbon-coating process in a CC7650 SEM Carbon Coater unit (Quorum Technologies Ltd, East Sussex, UK). Finally, SEM micrographs were registered, and a qualitative analysis was performed for the surface element distribution.

Disc-shaped specimens (10 mm in diameter and 2 mm high) were prepared and immersed in Hank’s Balanced Salt Solution (HBSS) for a total of 28 days. Following immersion, the samples were taken out and placed in acetone for 48 h, and dessicated in a vacuum desiccator for an additional 24 h. Subsequently, the surfaces of the samples were ground with progressively finer diamond discs using an automatic polishing machine. Both control and experimental groups were coated with carbon using a CC7650 SEM Carbon Coater Unit (Quorum Technologies Ltd.). The superficial surface morphology and the element distribution of the coated discs was individually examined at two time intervals of one day and 28 days, using a scanning electron microscopy (SEM) unit (Jeol 6100 EDAX; Jeol Inc.) at 150X magnification, coupled with an energy-dispersive spectroscopy (EDS) system (INCA 350 EDS; Oxford Instruments) for elemental analysis.