We purchased physcion (purity ≥ 98%, Figure 1 A) from Cayman Chemical Company (Ann Arbor, MI, USA); Isocove’s Modified Dulbecco’s Medium (IMDM) from Gibco BRL (Grand Island, NY, USA); fetal bovine serum (FBS) from Welgene (Daegu, Korea); DEX, PMACI, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), BAPTA-AM, Fura-2/AM, DNFB, and O-phthaldialdehyde from Sigma Chemical Co. (St. Louis, MO, USA); RPMI Media 1640 from Thermo Fisher Scientific Inc. (Waltham, MA, USA); anti-TSLP from R & D system Inc. (Minneapolis, MN, USA), IL-6 from Pharmingen (Sandiego, CA, USA), TNF-α (Pharmingen), IL-1β (R & D system Inc.), IL-4 (Pharmingen), IFN-γ (Pharmingen), and IgE antibodies (Pharmingen); anti- pERK, ERK, pJNK, JNK, pp38, p38, RIP2, caspase-1, pIKKβ, NF-κB, pIκBα, poly-ADP-ribose polymerase (PARP), GAPDH, and peroxidase-conjugated second antibodies from Santa Cruz Biotechnology (Santa Cruz, CA, USA); RIPA buffer from Cell signaling (Billerica, MA, USA).
Pmaci
Manufactured by Merck Group
Sourced in United States
PMACI is a laboratory equipment product manufactured by Merck Group. It serves as a platform for performing various analytical and experimental procedures in a controlled environment. The core function of PMACI is to provide a stable and reliable setting for conducting scientific investigations and analyses. No further details on intended use or interpretation of the product's capabilities are provided.
Automatically generated - may contain errors
Lab products found in correlation
Fura 2 am, by Merck Group (1 mentions)
Interleukin 4 (il 4), by BD (1 mentions)
Poly adp ribose polymerase parp, by Santa Cruz Biotechnology (1 mentions)
Isocove s modified dulbecco s medium imdm, by Thermo Fisher Scientific (1 mentions)
Anti perk, by BD (1 mentions)
Nf κb, by Santa Cruz Biotechnology (1 mentions)
P ikkβ, by Santa Cruz Biotechnology (1 mentions)
Rpmi 1640 media, by Thermo Fisher Scientific (1 mentions)
Caspase 1, by Santa Cruz Biotechnology (1 mentions)
O phthaldialdehyde, by Merck Group (1 mentions)
P iκbα, by Santa Cruz Biotechnology (1 mentions)
Ripa buffer, by Cell Signaling Technology (1 mentions)
Fetal bovine serum (fbs), by Welgene (1 mentions)
Interleukin 6 (il 6), by BD (1 mentions)
Tnf α, by BD (1 mentions)
Ifn γ, by BD (1 mentions)
Dexamethasone (dex), by Merck Group (1 mentions)
2 protocols using pmaci
1
Physcion-induced Immune Modulation and Signaling
2
Mast Cell Activation and Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HMC-1 cell (Cat# SCC067, RRID:CVCL_H206) was kindly provided by Eichi Morri (Osaka University, Osaka, Japan). HMC-1 cells were grown in IMDM supplemented with antibiotics (100 U/ml penicillin/100 μg/ml streptomycin) and 10% heat inactivated fetal bovine serum (FBS) at 37 °C, 5% CO2 and 95% humidity. HMC-1 cells were stimulated with 5 μM phorbol 12-myristate 13-acetate and 100 nM A23187 (PMACI, Sigma-Aldrich, St. Louis, Mo, USA) on 24 or 6 well plate. The following drugs were utilized to target phosphatidylinositide-3-kinase (PI3K), c-Jun NH2-terminal kinases (JNK), p38, extracellular signal-regulated kinase (ERK), activating protein (AP)-1, and nuclear factor (NF)-κB: Wortmannin, SP600125, SB203580, PD98059, SR11302, and pyrrolidine dithiocarbamate (PDTC). These drugs were obtained from Sigma-Aldrich. This study utilized 500 ng/ml of dexamethasone (Sigma-Aldrich) in accordance with the prior study [5] (link).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!