The NSCLC cells were pre-transfected with the above vectors, cultured in 96-well plates at standard culture conditions, and were subjected to cisplatin (25 μg/ml) stimulation for 0 h, 24 h, 48 h and 72 h, respectively. After that, the CCK-8 reaction solution (AbMole, USA) was incubated with the cells in the volume of 20 μl per well for 4 h at the incubator. Then, the plates were vortexed to thoroughly mix the cells with the solution, and were placed in a microplate reader (ThermoFisher Scientific, USA) to measure the optical density (OD) values at the wavelength of 450 nm, which could be used to represent relative cell proliferation in the cells.
Cck 8 reaction solution
Manufactured by AbMole
Sourced in United States
CCK-8 reaction solution is a colorimetric assay kit used to measure cell viability and cytotoxicity. It contains a water-soluble tetrazolium salt that is reduced by living cells, producing a colored formazan dye that can be quantified using a spectrophotometer.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using cck 8 reaction solution
1
Cell Proliferation Assay in NSCLC
2
Cisplatin-Induced NSCLC Cell Viability
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The NSCLC cells were pre-transfected with the above vectors, cultured in 96-well plates at standard culture conditions, and were subjected to cisplatin (25 μg/ml) stimulation for 0 h, 24 h, 48 h and 72 h, respectively. After that, the CCK-8 reaction solution (AbMole, USA) was incubated with the cells at the volume of 20 μl per well for 4 h at the incubator. Then, the plates were shattered to thoroughly mix the cells with the solution, and were placed in a microplate reader (ThermoFisher Scienti c, USA) to measure the optical density (OD) values at the wavelength of 450 nm, which could be used to represent relative cell proliferation in the cells.
3
Cisplatin-Induced NSCLC Cell Proliferation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The NSCLC cells were pre-transfected with the above vectors, cultured in 96-well plates at standard culture conditions, and were subjected to cisplatin (25 μg/ml) stimulation for 0 h, 24 h, 48 h and 72 h, respectively. After that, the CCK-8 reaction solution (AbMole, USA) was incubated with the cells in the volume of 20 μl per well for 4 h at the incubator. Then, the plates were vortexed to thoroughly mix the cells with the solution, and were placed in a microplate reader (ThermoFisher Scienti c, USA) to measure the optical density (OD) values at the wavelength of 450 nm, which could be used to represent relative cell proliferation in the cells.
4
Cisplatin-Induced NSCLC Cell Viability
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The NSCLC cells were pre-transfected with the above vectors, cultured in 96-well plates at standard culture conditions, and were subjected to cisplatin (25 μg/ml) stimulation for 0 h, 24 h, 48 h and 72 h, respectively. After that, the CCK-8 reaction solution (AbMole, USA) was incubated with the cells at the volume of 20 μl per well for 4 h at the incubator. Then, the plates were shattered to thoroughly mix the cells with the solution, and were placed in a microplate reader (ThermoFisher Scienti c, USA) to measure the optical density (OD) values at the wavelength of 450 nm, which could be used to represent relative cell proliferation in the cells.
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