NBQX disodium salt (2,3-Dioxo-6-nitro-1,2,3,4-tetrahydrobenzo[f]quinoxaline-7-sulfonamide disodium salt) (Tocris Bioscience) was dissolved in 0.9% saline and administered intraperitoneally (i.p.) at doses of 0.0 (vehicle control), 1.0, 2.0, 4.0, 6.0, and 8.0 mg/kg. THIP hydrochloride (4,5,6,7-Tetrahydroisoxazolo[5,4-c]pyridine-3-ol hydrochloride) (Tocris Bioscience) was dissolved in 0.9% saline, and administered i.p. at doses of 0.0 (vehicle control), 1.0, 2.0 and 4.0 mg/kg. The non-competitive NMDA receptor antagonist ifenprodil hemitartrate ((1R*,2S*)-erythro-2-(4-Benzylpiperidino)-1-(4-hydroxyphenyl)-1-propanol hemi-(DL)-tartrate) (Tocris Bioscience) was dissolved in double-distilled sterile water (ddH2O) and administered i.p. at doses of 0 (vehicle control), 1.5, 3.0 and 6.0 mg/kg. All doses were administered at a volume of 2 ml of drug solution/kg body weight.
Thip hydrochloride
Manufactured by Bio-Techne
Sourced in United Kingdom
THIP hydrochloride is a chemical compound used as a research tool in various scientific applications. It functions as a potent and selective agonist for the GABAA receptor.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using thip hydrochloride
1
Dose-Dependent Pharmacological Interventions
2
Dose-Dependent Effects of AMPA and GABAA Receptor Modulators
3
Pharmacological Modulation of Sleep in Rats
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Zolpidem (Tocris Bioscience, UK) was dissolved in 10% Ethanol, 20% polyethylene glycol, and 70% distilled water at a final concentration of 3 mg/ml. Diazepam (Tocris Bioscience, UK) was dissolved in 10% dimethyl sulfoxide, 20% Cremophor EL and 70% saline at a final concentration of 4 mg/ml. THIP hydrochloride (Tocris Bioscience, UK) was dissolved in saline at a final concentration of 4 mg/ml. Each drug and their respective vehicles were intraperitoneally (i.p.) administered at a volume of 1 ml/kg in counterbalanced order across rats. Control experiments were undertaken with both saline and each drug’s respective vehicle; neither saline nor vehicles had any significant effects on neural activity, so only saline experiments are shown here.
Doses were optimized on the basis of previous studies: 3 mg/kg Zolpidem has been shown to increase sleep duration and SWA in rats [55 (link)] and reaches peak plasma concentration within 15 min, with a half-life of approximately 20 min [56 ]; 4 mg/kg Diazepam caused sedation as well as a decrease in SWA in rodents [57 (link)–59 (link)], again achieving peak concentration within 10 min of injection and clearing with a half-life in the order of 1 h [60 ]; 4 mg/kg of THIP has been shown to increase NREM sleep and enhance SWA in rats [61 (link)].
Doses were optimized on the basis of previous studies: 3 mg/kg Zolpidem has been shown to increase sleep duration and SWA in rats [55 (link)] and reaches peak plasma concentration within 15 min, with a half-life of approximately 20 min [56 ]; 4 mg/kg Diazepam caused sedation as well as a decrease in SWA in rodents [57 (link)–59 (link)], again achieving peak concentration within 10 min of injection and clearing with a half-life in the order of 1 h [60 ]; 4 mg/kg of THIP has been shown to increase NREM sleep and enhance SWA in rats [61 (link)].
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