Retinas were isolated from enucleated eyes, incubated with 0.25% Trypsin for 15 minutes. Then, retinas were treated with 20% fetal bovine serum and DNaseI, dissociated into single cells and stained with PE conjugated anti-Cd73 antibody (BD Pharmingen) for 30 minutes on ice. Retinas were washed with 2% bovine serum albumin and stained with propidium iodide. Cd73 negative and positive cells were collected using FACSAria II (BD).
Pe conjugated anti cd73 antibody
Manufactured by BD
Sourced in United States
The PE conjugated anti-CD73 antibody is a laboratory tool used for the identification and analysis of CD73-expressing cells. CD73 is an enzyme involved in the regulation of extracellular adenosine levels. This antibody is conjugated to the fluorescent dye phycoerythrin (PE), enabling the detection and quantification of CD73-positive cells through flow cytometry or other immunoassay techniques.
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2 protocols using pe conjugated anti cd73 antibody
1
Isolation and Characterization of CD73+ Retinal Cells
2
Mesenchymal Marker Expression in hMSCs
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The expression of mesenchymal markers was analyzed by flow cytometry in hMSCs (T = 0 h), hMSCs-derived adipocytes (T = 20 day), and hypertrophic-like adipocytes (T = 32 day). The cells were incubated with PE-conjugated anti-CD73 antibody and FITC-conjugated anti-CD90 antibody, as well as with dye/isotype-matched antibodies (all from BD Biosciences, USA). The incubation was carried for 30 min at 4 °C in a dark environment. Afterward, unbound antibodies were washed out and the samples were processed by a BD FACS canto II (BD Biosciences, San Jose, CA) and analyzed using BD FACSDiva software. For each sample, 104 events were acquired. Cells were counted and compared with the signals of the corresponding antibody isotype controls.
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