Part of the S2 fraction was filtered through a 0.45 µm syringe filter to remove particles. The filtrate was diluted in MilliQ water to reach a protein concentration of around 1 mg/mL (Wprot/V), transferred to a disposable capillary zeta cell (Malvern Instruments, Malvern, UK), and analyzed with a Zetasizer nano ZS (Malvern Instruments, Malvern, UK) coupled with an autotitration unit (MPT-2, Malvern). The pH of the solution was changed in steps of 0.5 units using 0.1 and 0.01 M HCl, and particle size and their zeta potential (ZP) were measured at each step. Duplicate measurements were made for two process replicates, and at each pH value triplicate particle size and ZP measurements were averaged.
Disposable capillary zeta cell
Manufactured by Malvern Panalytical
Sourced in United Kingdom
The Disposable capillary zeta cell is a laboratory instrument used to measure the zeta potential of samples. It is a single-use, disposable cell that is designed to hold the sample for analysis. The cell has a capillary design that allows for accurate and reliable zeta potential measurements.
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Lab products found in correlation
2 protocols using disposable capillary zeta cell
1
Zeta Potential and Particle Size Analysis
2
Zeta Potential and Isoelectric Point of Hydrolyzed Wheat Germ Proteins
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The zeta potential of supernatants 3 (see Figure 7.1) of unheated DH 6 WGPs obtained by hydrolysis with different enzymes as described above was measured in a Zetasizer Nano ZS (Malvern Instruments, Worcestershire, UK) instrument at 20 °C based on laser Doppler micro-electrophoresis. To this end, duplicates of aqueous solutions (2.0 protein/ml) were transferred to a disposable capillary zeta cell (Malvern Instruments) in which an electric potential was applied. In this type of measurement, the charge of the peptides results in specific electrophoretic mobility, which then is converted to a zeta potential with the Henry equation. In addition, the isoelectric point of supernatants 1 (see Figure 7.1) of unheated tryptic DH 2 and DH 6 WGPs was determined as the pH at which their zeta potential was zero. For this, the zeta potential of aqueous solution (1.5 mg protein/ml) contained in a similar cell was monitored as a function of pH changes resulting from adding 0.25 M or 0.50 M hydrochloric acid (HCl) using the autotritrator device of the Zetasizer Nano ZS.
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