Liquid chromatography‒mass spectrometry (LC/MS)-grade acetonitrile was obtained from Fisher (Thermo Fisher Scientific, Waltham, MA, USA). Purified water was purchased from Wahaha (Hangzhou, China). Ammonium acetate, ammonium formate, diethyl ether and hydroxypropyl-β-cyclodextrin were purchased from Sinopharm (Beijing, China). Acetic acid was obtained from TEDIA (Fairfield, USA). Formic acid was provided by Millipore (Merck, Burlington, MA, USA), and 0.9% NaCl was obtained from CSPC (Shijiazhuang, China). Histamine, γ-aminobutyric acid (GABA), 1-methyhistamine, decynium 22, S-adenosyl-l-methionine, and semicarbazide (SCZ) were purchased from Sigma–Aldrich (St. Louis, MO, USA). Histamine-α,α,β,β-d4·2HCl was purchased from CDN isotopes (Quebec, Canada). [U–13C]glucose and isotopic histidine (Ring-2-13 (link)C) were obtained from Cambridge Isotope Laboratories, Inc. (MA, USA). 4-Aminobutyric-2,2-d2 acid (GABA-d2) was purchased from ZZBIO Co., Ltd. (Shanghai, China). Corticosterone, β-estradiol, 2-amino-2-norbornanecarboxylic acid (BCH), and amodiaquine dihydrochloride were purchased from MedChemExpress (Shanghai, China). YZG-331 was kindly provided by Professor Jiangong Shi (Institute of Materia Medica, Chinese Academy of Medical Sciences (CAMS), Beijing, China).
Corticosterone
Manufactured by MedChemExpress
Sourced in China
Corticosterone is a laboratory equipment product used for research purposes. It is a steroid hormone that plays a key role in the regulation of various physiological processes.
Automatically generated - may contain errors
Lab products found in correlation
Semicarbazide, by Merck Group (1 mentions)
Ammonium formate, by Sinopharm (1 mentions)
Formic acid, by Merck Group (1 mentions)
Amodiaquine dihydrochloride, by MedChemExpress (1 mentions)
β estradiol, by MedChemExpress (1 mentions)
Decynium 22, by Merck Group (1 mentions)
S adenosyl l methionine, by Merck Group (1 mentions)
Lc ms grade acetonitrile, by Thermo Fisher Scientific (1 mentions)
Acetic acid, by Tedia (1 mentions)
Ammonium acetate, by Sinopharm (1 mentions)
Diethyl ether, by Sinopharm (1 mentions)
Lps l5293, by Merck Group (1 mentions)
Dmem f12 medium, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Lipopolysaccharides (lps), by MedChemExpress (1 mentions)
2 protocols using corticosterone
1
Targeted Metabolomic Profiling LC-MS Workflow
2
Cell Stress Model and Pharmacological Intervention
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The PC-12 cell line was purchased from the National Collection of Authenticated Cell Cultures (China, Shanghai) and the HAPI cell line from BeNa Culture Collection (Beijing, China). PC-12 cells were cultured in RPMI 1640 medium (Gibco, USA) and the HAPI cells in the DMEM/F-12 medium (Gibco). All culture systems were supplemented with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin (Gibco) under a humidified atmosphere of 5% CO2 at 37 °C.
The cell treatment of the experiments is depicted in Figs.6 and 7 and was as follows: for the LPS/corticosterone + Rg1 group, the cells were pre-treated with LPS (1 μg/mL, to establish an inflammatory stress model) or corticosterone (400 μM, to establish an oxidative stress model) for 2 h, followed by co-incubation with Rg1 (5 μM, 10 μM, 20 μM) for 24 h. For the LPS/corticosterone + shRNA group, the cells were transfected with lentivirus for 48 h and then washed with cold PBS buffer, followed by incubation with LPS or corticosterone for 24 h. corticosterone (HY-B1618) was purchased from MedChemExpress and LPS (L5293) from Sigma-Aldrich.
The cell treatment of the experiments is depicted in Figs.
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