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Biotin sp conjugated donkey anti goat igg

Manufactured by Jackson ImmunoResearch
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Biotin-SP-conjugated donkey-anti-goat IgG is a secondary antibody conjugate produced by Jackson ImmunoResearch. It is designed to recognize and bind to goat immunoglobulin G (IgG) molecules. The biotin-SP (long-spacer) conjugation allows for versatile detection and amplification applications.

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Lab products found in correlation

Peroxidase conjugated streptavidin, by Jackson ImmunoResearch (2 mentions) Goat anti rabbit rtla, by Cedarlane (2 mentions) 3 3 diaminobenzide, by Merck Group (2 mentions) Hematoxylin qs, by Vector Laboratories (2 mentions) Donkey anti mouse igg, by Jackson ImmunoResearch (2 mentions)

Spelling variants of this product

Donkey anti-goat IgG (29 citations) Biotin-conjugated donkey anti-goat IgG (3 citations) Biotin-anti-IgG (2 citations)

2 protocols using biotin sp conjugated donkey anti goat igg

1

Immunophenotyping of T Cells and Bone Marrow Cells

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OCT embedded tissues were cut at ~8-10 μm, fixed with paraformaldehyde/zinc; blocked with Aurion® blocking solution containing normal donkey serum; and incubated either with goat anti-rabbit RTLA (Rabbit TLymphocyte Antigen, Cedarlane Labs, Burlington, NC) as a marker for T cells or with mouse anti-rabbit CD18 (Antigenix America, Huntington Station, NY) as a marker for bone marrow-derived cells. Biotin-SP-conjugated donkey-anti-goat IgG, donkey anti-mouse IgG, and peroxidase-conjugated streptavidin were from Jackson ImmunoResearch Laboratories (West Grove, PA). 3,3′diaminobenzide was from Sigma-Aldrich (St. Louis, MO). Sections were counterstained with Hematoxylin QS® (Vector Laboratories, Burlingame, CA). The percentages of total cells immunopositive for RTLA and CD18 were determined with MetaMorph® Microscopy Automation and Image Analysis Software.
Mircheff A.K., Wang Y., Schechter J.E., Li M., Tong W., Attar M., Chengalvala M., Harmuth J, & Prusakiewicz J.J. (2016). Multiple Natural and Experimental Inflammatory Rabbit Lacrimal Gland Phenotypes. The ocular surface, 14(4), 460-483.e3.
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2

Immunophenotyping of T Cells and Bone Marrow Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
OCT embedded tissues were cut at ~8-10 μm, fixed with paraformaldehyde/zinc; blocked with Aurion® blocking solution containing normal donkey serum; and incubated either with goat anti-rabbit RTLA (Rabbit TLymphocyte Antigen, Cedarlane Labs, Burlington, NC) as a marker for T cells or with mouse anti-rabbit CD18 (Antigenix America, Huntington Station, NY) as a marker for bone marrow-derived cells. Biotin-SP-conjugated donkey-anti-goat IgG, donkey anti-mouse IgG, and peroxidase-conjugated streptavidin were from Jackson ImmunoResearch Laboratories (West Grove, PA). 3,3′diaminobenzide was from Sigma-Aldrich (St. Louis, MO). Sections were counterstained with Hematoxylin QS® (Vector Laboratories, Burlingame, CA). The percentages of total cells immunopositive for RTLA and CD18 were determined with MetaMorph® Microscopy Automation and Image Analysis Software.
Mircheff A.K., Wang Y., Schechter J.E., Li M., Tong W., Attar M., Chengalvala M., Harmuth J, & Prusakiewicz J.J. (2016). Multiple Natural and Experimental Inflammatory Rabbit Lacrimal Gland Phenotypes. The ocular surface, 14(4), 460-483.e3.
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