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Sodium hyaluronate ha

Manufactured by Merck Group
Sourced in United States

Sodium hyaluronate (HA) is a naturally occurring polysaccharide found in the human body. It is a key component of the extracellular matrix and plays a crucial role in various physiological processes. HA is a linear polymer composed of repeating disaccharide units of D-glucuronic acid and N-acetyl-D-glucosamine, linked by β-1,4 and β-1,3 glycosidic bonds.

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3 protocols using sodium hyaluronate ha

1

Aptamer-Mediated Targeted Delivery of Lipid Nanoparticles

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DSPC (Avanti Corporation, USA), DSPE-PEG2000 (Avanti Corporation, USA), DSPE-PEG2000-maleimide (Avanti Company, USA), DC-cholesterol∙HCl (Avanti Company, USA), DPPE (Avanti Company, USA), glycerol (Sigma, USA), sodium hyaluronate (HA, 200–400 kDa, Xi’an Ruixi), aptamer PM1 base sequence SH-CCCCTGCAGGTGATTTTGCTCAAGTCGTTCCCGTCCTCTCCTCTGCGCCCCGTCTCGGCCAGTATCGCTAATCAGGCGGAT-HS, (Shanghai Shenggong, China), a sequence with FITC-labelled or unlabelled at the 5ʹ end, ANM33 and ANM33 double-modified with cy3 and amino groups (Guangzhou Ruibo, China), DiI fluorescent dye (Yeasen, USA), RAW264.7-T1 macrophages (Procell Life Science & Technology, China), oxidized low-density lipoprotein (ox-LDL; Yiyuan Biotechnology, China), Oil red O staining kit (Sigma, China), high-glucose DMEM (ScienCell, China), and double antibodies (ScienCell, USA) were used. A fluorescence microscope (LEICA CTR6000, Germany), laser confocal microscope (Leica SP8, Germany), flow cytometer (Beckman, USA), and laser nanoparticle size and zeta potential analyser (Malvern, UK) were also used.
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2

Development of Epsiliseen®-H Hydrogel Formulations

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Epsiliseen®-H (εPL, ε-polylysine, MW 4.7 kDa, Food Grade) was purchased from Siveele B.V. (Breda, Netherlands). Trans-ferulic acid (FA, MW 194.18 Da), Pluronic® F68 (poloxamer 188, PF68, MW 8.4 kDa), polyvinylpyrrolidone (PVP, MW 360 kDa), sodium hyaluronate (HA, MW 600–1100 kDa) and Tryptan blue powder were from Sigma Aldrich (St. Louis, MO, USA). Polyethylene oxide (PEO, MW 200 kDa) was purchased from Thermo Fisher Scientific (Waltham, MA, US). Ethanol absolute AnalaR Normapur® (EtOH, Reagent Ph Eur, Reagent USP) was purchased from VWR Chemicals (Milano, Italia). Water was purified using reverse osmosis (resistivity > 18 MΩ cm, MilliQ, Millipore®, Madrid, Spain). Supragradient HPLC grade acetonitrile and acetic acid glacial (reagent grade) were from Scharlau Chemicals (Barcelona, Spain). NaCl was from Panreac Quimica S.L.U. (Barcelona, Spain).
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3

Polymer-based Hyaluronate Formulation

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Sodium hyaluronate (HA) and methylcellulose (MC) were purchased in Sigma-Aldrich (St. Louis, MO, USA). HA was obtained from rooster comb and was expected to have a molecular weight of 1–4 million. Polyacrylic acid (PA) was supplied from Lubrizol (Brumach, Belgium). Sodium hydroxide and potassium dihydrogen phosphate anhydrous were provided by Chempur (Piekary Śląskie, Poland). The phosphate buffer solution pH = 7.4 was prepared according to European Pharmacopoeia 10.5 [16 ].
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