Anti pot1 nb500 176

The ethyl alcohol (pure (E7023) and FastStart Essential DNA Green Master (06402712001)) were obtained from Sigma-Aldrich (Darmstadt, Germany). The 16% formaldehyde (methanol-free) was obtained from Thermo Fisher Scientific (28906, Seoul, Republic of Korea). The μ-Dish 35mm image dishes with polymer coverslip bottoms were obtained from ibidi GmbH (Gräfelfing, Germany). N-acetyl-L-cysteine (A7250) and MitoTEMPO (SML0737) were obtained from Sigma-Aldrich (Darmstadt, Germany).
The antibodies against RAP1 were obtained from Bethyl laboratories, Inc. (A300-306A-9, MA, USA); the anti-TPP1 (ab195234), anti-TRF1 (ab10579), anti-p16 (ab211542), and anti-53BP1 (ab175933) were from Abcam (Cambridge, UK); the anti-POT1 (NB500-176) and anti-TRF2 (NB100-56506) were from Novus Biologicals (Littleton, CO, USA); the anti-TIN2 (PA5-67076) was from Invitrogen (Waltham, MA, USA); the anti-p53 (P6874), p16 (ab211542), and anti-actin (A5441) were from Sigma-Aldrich (Darmstadt, Germany); and the anti-P21 (2947), PINK1 (6946), Parkin (4211), autophagy sampler kit (4445), SirT3 (2627), SirT5 (8779), and Tom20 (42406) were obtained from Cell Signaling Technology (Danvers, MA, USA). Ginsenoside F1 was prepared as described in our previous report [34 (link)].

ChIP assays were performed as previously described (Wu et al., 2014 (link)). Antibodies used were as follows: anti-cGAS (CST), anti-CDK1 (Abcam), anti-TRF1 (GTX77605, GeneTex), anti-TRF2 (05-521, Millipore), anti-POT1 (NB500-176, Novus), anti-IgG (Millipore).
ChIP DNA were subjected to slot blot and hybridization with biotin-labeled telomeric G-rich probe or Alu probe. Probe sequences are: Telo probe 5′-TTAGGGTTAGGGTTAGGGT-3′; Alu probe 5′-GGCCGGGCGCGGTGGCTCACG CCTGTAATCCCAGCA-3′. ChIP DNA were also used for q-PCR to determine subtelomeric DNA. Primers used were listed in Table S1.