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1 palmitoyl 2 oleoyl sn glycero 3 phospho 1 rac glycerol

Manufactured by Merck Group

1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) is a synthetic phospholipid. It is a component used in the formulation of various lab equipment and materials.

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2 protocols using 1 palmitoyl 2 oleoyl sn glycero 3 phospho 1 rac glycerol

1

Lipid-Dependent Enzyme Activity Assay

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In the in vitro assays testing the dependence of the activities of various enzymes on specific lipids, the following synthetic lipid molecules (Merck) were added to the reaction mixture: 1-palmitoyl-2-oleoyl-sn-glycerol (DAG) (catalogue number 800815), N-octanoyl-d-erythro-sphingosine (CER) (catalogue number 860645), N-palmitoyl-d-erythro-sphinganine (dhCER) (catalogue number 860634), 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (PG) (catalogue number 810218), and 1,3-dihexadecanoyl-2-(cis-9-octadecenoyl)glycerol (TAG) (catalogue number D2157). Lipids were solubilized in chloroform-methanol (2:1, vol/vol). Before use, the required amount of lipids in the solution was added to a Pyrex tube and dried under a stream of nitrogen. The dried lipids were resuspended in the appropriate buffer described above.
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2

In Vitro Lipid Enzyme Assays

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In the in vitro assays testing the dependence of activity of various enzymes on specific lipids, the following synthetic lipid molecules (Merck) were added to the reaction: 1-palmitoyl-2-oleoyl-sn-glycerol (DAG; Cat. #800815), N-octanoyl-Derythro-sphingosine (CER; Cat. #860645), N-palmitoyl-D-erythro-sphinganine (dhCER, Cat. #860634), 1-palmitoyl-2-oleoylsn-glycero-3-phospho-(1′-rac-glycerol) (PG; Cat. #810218), 1,3-dihexadecanoyl-2-(cis-9-octadecenoyl)glycerol (TAG, Cat. # D2157). Lipids were solubilized in chloroform:methanol (2:1, v:v). Before use, the required amount of lipids in the solution was added to the pyrex tube and dried under a stream of nitrogen. The dried lipids were resuspended in the appropriate buffer described above.
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