Compounds were stored desiccated at -20 °C. Quinolones were dissolved in autoclaved HPLC-grade water or NaOH (both JT Baker, Phillipsburg, NJ) solution; etoposide (Sigma, St. Louis, MO) was dissolved in DMSO (Sigma, St. Louis, MO). Tested were: ciprofloxacin HCl, clinafloxacin (Santa Cruz Biotechnology, Santa Cruz, CA); potassium fleroxacin (Hoffman-La Roche, Basel, Switzerland); gatifloxacin (Selleck Chemicals, Houston, TX); levofloxacin, sodium nalidixate, oxolinic acid, piromidic acid (Sigma, St. Louis, MO); norfloxacin (Merck Sharpe & Dohme, Whitehouse Station, NJ); ofloxacin (Robert Wood Johnson Pharm, NJ); and pefloxacin methanesulfonate (Rhône Poulenc Rorer now Sanofi-Aventis, Bridgewater, NJ).
Sodium nalidixate
Manufactured by Merck Group
Sodium nalidixate is a chemical compound used as a laboratory reagent. It serves as a source of sodium ions and is commonly used in various analytical and experimental applications in research and laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
Oxolinic acid, by Merck Group (1 mentions)
Levofloxacin, by Merck Group (1 mentions)
Ciprofloxacin hcl, by Santa Cruz Biotechnology (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Etoposide, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Avantor (1 mentions)
Novobiocin, by Merck Group (1 mentions)
2 protocols using sodium nalidixate
1
Quinolones Compound Storage and Dissolution
2
Pathogenicity of Salmonella Serovars in Chickens
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Two-day-old Lohmann Lite chickens were purchased from the Millennium Hatchery (Birmingham, UK). Two groups, each with 12 birds, were inoculated orally with 108 CFU of S. Enteritidis or S. Gallinarum in 0.1 mL of nutrient broth. Another 12 birds inoculated orally with 0.1 mL of PBS were in the control group. Three groups in separate pens were given access to antibiotic-free feed and water ad libitum throughout the experiment. At 1, 2, 4, and 5 days post-inoculation, three birds from each group were sacrificed. Caecal contents and liver were collected aseptically and processed to determine bacterial counts using Brilliant Green agar plates containing sodium nalidixate (20 μg/mL; Sigma-Aldrich) and novobiocin (1 μg/mL; Sigma-Aldrich). Spleen and cecal tonsils were collected for cytokine mRNA expression analysis [18 (link)]. All animal care and experimentation were carried out under Home Office project license PPL 40/3412 and had local ethical approval from the University of Nottingham Animal Welfare and Ethical Review Body, April 2015.
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