C57BL/6 J mice were purchased from the Shanghai SLAC Laboratory Animal Co.,Ltd; CD1d-deficient and IL-17A-deficient mice were obtained from the Jackson Laboratory. All the above mice were kept under the specific pathogen-free conditions and the study was approved by Experimental Animal Ethics Committee of the First Affiliated Hospital, Zhejiang University (No. 2021–0019-1).
Il 17a deficient mice
Manufactured by Jackson ImmunoResearch
IL-17A-deficient mice are genetically engineered mice that lack the gene responsible for the production of the cytokine interleukin-17A (IL-17A). These mice can be used as a research tool to study the role of IL-17A in various biological processes and disease models.
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Lab products found in correlation
2 protocols using il 17a deficient mice
1
Investigating Immune Responses in Mice
2
Mouse Model of Serum Transfer Arthritis
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Animal procedures were performed in accordance with protocol A-2262 approved by the IACUC at the University of Massachusetts Medical School. KRN T cell transgenic mice (provided by Drs. Benoist and Mathis, Harvard Medical School and Institut de Genetique et de Biologie Moleculaire et Cellulaire, Illkirch, France) were crossed with NOD mice (Jackson) to generate arthritic K/BxN mice [24 (link), 25 (link)]. Arthritogenic serum was prepared from 9-week-old K/BxN mice. STA was induced in 12-week-old male IL-17A-deficient mice (Jackson). Absence of IL-17A expression was confirmed by intracellular staining (personal communication, Dr. Keiji Hirota). These mice were backcrossed onto the C57BL/6 background for six generations and C57BL/6J mice (wild-type) were used as controls. Arthritogenic serum, 150 μl per intraperitoneal injection, was administered on days 0, 2, and 7. Ankle thickness was measured as an indicator of inflammation and clinical inflammation was scored as previously described [26 (link)].
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