Nci h1734

Manufactured by American Type Culture Collection

Sourced in United States

The NCI-H1734 is a cell line derived from a human lung adenocarcinoma. It is a commonly used model for studying lung cancer and related research.

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H1734 (5 citations)

5 protocols using nci h1734

Hypoxia Exposure of NSCLC Cells

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Human NSCLC cells (NCI-H1650, NCI-H1385, NCI-H460, NCI-H522, NCI-H661, NCI-H2126, NCI-H23, NCI-H1703, NCI-H1435, NCI-H596, NCI-H2286, NCI-H1437, NCI-H1651, NCI-H2085, NCI-H2342, NCI-H2073, NCI-H1793, NCI-H2170, NCI-H1299, NCI-H2066, NCI-H2347, NCI-H1734, NCI-H1563, NCI-H441, NCI-H1975, A549, Calu-3, NCI-H2228) were purchased from ATCC (Manassas, VA). Cells were maintained in the respective medium supplemented with 10% v/v FBS, 1% v/v penicillin-streptomycin, 1% v/v L-glutamine, in a Heracell incubator (ThermoFisher, Waltham, MA) with different pO₂. The experimental conditions were set as it followed: 24 h at 20% O₂ (normoxia), 24 h at 1% O₂ (hypoxia), 12 h at 1% O₂ followed by 12 h at 20% O₂ (hypoxia/normoxia), 12 h at 1% O₂ followed by 12 h at 20% O₂ and 12 h at 1% O₂ (hypoxia/normoxia/hypoxia or intermittent hypoxia).

Salaroglio I.C., Belisario D.C., Akman M., La Vecchia S., Godel M., Anobile D.P., Ortone G., Digiovanni S., Fontana S., Costamagna C., Rubinstein M., Kopecka J, & Riganti C. (2022). Mitochondrial ROS drive resistance to chemotherapy and immune-killing in hypoxic non-small cell lung cancer. Journal of Experimental & Clinical Cancer Research : CR, 41, 243.

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Fluorescent Lung Cancer Cell Lines

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A-549 (CCL-185), NCI-H1299 (CRL-5803), NCI-H2228 (CRL-5935), NCI-H460 (HTB-177), NCI-H1734 (CRL-5891), NCI-H1993 (CRL-5909), and HCC827 (CRL-2868) cells were from ATCC. PC-9 cells were from Sigma-Aldrich. NCI-H3122 cells were from the National Cancer Institute. All cell lines were transduced with the FU-EBFP2-H2B-W plasmid that expresses a nuclear-localized blue-fluorescent protein. All cell lines were cultured in RPMI1640 supplemented with FBS (10% v/v), glutamine (4 mM), and penicillin/streptomycin (100 U/mL). PC9, H1229, and H3122 cell lines were also cultured in HPLM supplemented with FBS (10% v/v) and penicillin/streptomycin (100 U/mL).

Ghezzi C., Perez S., Ryan K., Wong A., Chen B.Y., Damoiseaux R, & Clark P.M. (2022). Early reduction of glucose consumption is a biomarker of kinase inhibitor efficacy which can be reversed with GLUT1 overexpression in lung cancer cells. Molecular imaging and biology, 25(3), 541-553.

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Hypoxia Modulation of NSCLC Cell Lines

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Human NSCLC cells (NCI-H1650, NCI-H1385, NCI-H460, NCI-H522, NCI-H661, NCI-H2126, NCI-H23, NCI-H1703, NCI-H1435, NCI-H596, NCI-H2286, NCI-H1437, NCI-H1651, NCI-H2085, NCI-H2342, NCI-H2073, NCI-H1793, NCI-H2170, NCI-H1299, NCI-H2066, NCI-H2347, NCI-H1734, NCI-H1563, NCI-H441, NCI-H1975, A549, Calu-3, NCI-H2228) were purchased from ATCC (Manassas, VA). Cells were maintained in the respective medium supplemented with 10% v/v FBS, 1% v/v penicillin-streptomycin, 1% v/v L-glutamine, in a Heracell incubator (ThermoFisher, Waltham, MA) with different pO 2 . The experimental conditions were set as it followed: 24h at 20% O 2 (normoxia), 24h at 1% O 2 (hypoxia), 12 h at 1% O 2 followed by 12h at 20% O 2 (hypoxia/normoxia), 12h at 1% O 2 followed by 12h at 20% O 2 and 12h at 1% O 2 (hypoxia/normoxia/hypoxia or intermittent hypoxia).

Salaroglio I.C., Belisario D.C., Akman M., Vecchia S.L., Godel M., Anobile D.P., Ortone G., Digiovanni S., Fontana S., Costamagna C., Kopecka J., & Riganti C. (2022). C/EBP-β LAP regulates chemo-immuno-resistance in hypoxic non-small cell lung cancer.

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Lung Cancer Cell Line Culture and Maintenance

Cited 1 time

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Human lung cancer cell lines, A549, NCI-H1734, NCI-H1792, NCI-H441, NCI-H23, NCI-H1975 and NCI-H520 cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). Normal human tracheobronchial epithelial (NHTBE) cells were obtained from the Lonza (Walkersville, MD, USA). Cell lines were passaged for less than 6 months following resuscitation and were not authenticated. All cancer cell lines were maintained under 5% CO₂ at 37°C in RPMI-1640 medium (Life Technologies, Carlsbad, CA, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Sigma-Aldrich, St. Louis, MO, USA) and 1% Antibotic-Antimycotic (Anti-Anti, Life Technologies). NHTBE cells were cultured in BEBM supplemented with growth factors and hormones provided by manufactory (Lonza), and three-demensional organotypic air-liquid interface (ALI) cell culture method was utilized for NHTBE cell culture, as described previously [5 (link), 17 (link)–19 (link)]. HEK293T cells were maintained in DMEM medium supplemented with 10% FBS and 1% Anti-Anti.

Lee J.W., Park H.S., Park S.A., Ryu S.H., Meng W., Jürgensmeier J.M., Kurie J.M., Hong W.K., Boyer J.L., Herbst R.S, & Koo J.S. (2015). A Novel Small-Molecule Inhibitor Targeting CREB-CBP Complex Possesses Anti-Cancer Effects along with Cell Cycle Regulation, Autophagy Suppression and Endoplasmic Reticulum Stress. PLoS ONE, 10(4), e0122628.

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NSCLC Cell Line Panel for Research

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The human NSCLC cell lines HCC827, NCI‐H1437, NCI‐H1734, NCI‐H358, NCI‐H1781, NCI‐H2170, NIC‐H1650, NCI‐H2106, NCI‐H2087, NCI‐H2347, NCI‐H441, Hs 618.T, NCI‐H1299, NCI‐H460, NCI‐H1975, NCI‐H1568, NCI‐H23, Calu‐3, and A549 were obtained from the American Type Culture Collection (Manassas, VA, USA). The human NSCLC cell line COR‐L105 was purchased from Sigma‐Aldrich, and human NSCLC cell line HCC‐15 was purchased from Creative Dynamics (Shirley, NY , USA). The HS618.T cell line was cultured in Dulbecco's Modified Eagle's medium, supplemented with 10% FBS. A549 was cultured in F‐12K medium, supplemented with 10% FBS. NCI‐H2106 was cultured in HITES medium supplemented with 5% FBS. Calu‐3 was cultured in Eagle's Minimum Essential Medium supplemented with 10% FBS. NCI‐H2087 was cultured in RPMI‐1640 medium supplemented with 5% FBS. All other cell lines were maintained in RPMI‐1640 medium supplemented with 10% FBS. Cells were grown at 37 °C in a humidified 5% CO₂ : 95% air atmosphere.

Tavassoly I., Hu Y., Zhao S., Mariottini C., Boran A., Chen Y., Li L., Tolentino R.E., Jayaraman G., Goldfarb J., Gallo J, & Iyengar R. (2019). Genomic signatures defining responsiveness to allopurinol and combination therapy for lung cancer identified by systems therapeutics analyses. Molecular Oncology, 13(8), 1725-1743.

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